Studying the synergistic damage effects induced by 1.8 GHz radiofrequency field radiation (RFR) with four chemical mutagens on human lymphocyte DNA using comet assay in vitro.
Baohong Wang, Jiliang H, Lifen J, Deqiang L, Wei Z, Jianlin L, Hongping D. · 2005
View Original Abstract1.8 GHz radiofrequency field radiation alone did not damage human lymphocyte DNA, but it enhanced damage caused by two of four chemical mutagens tested.
Plain English Summary
Researchers studied whether 1.8 GHz radiofrequency field radiation (RFR) at a SAR of 3 W/kg could interact with four chemical mutagens to damage DNA in human lymphocytes in vitro. DNA damage was detected using comet assay at two incubation times after treatment. RFR alone showed no difference in DNA damage compared to the control group, but co-exposure with MMC or 4NQO produced significantly greater DNA damage than those chemicals alone. Co-exposure with bleomycin or methyl methanesulfonate did not significantly increase DNA damage compared with those chemicals alone.
Why This Matters
Tail length and tail moment were used as the DNA damage indexes. Cells were exposed to RFR and chemical mutagens for 2 and 3 hours respectively, and damage was assessed at both 0 and 21 hours after treatment. The four chemicals tested represent different mechanisms of DNA damage: MMC is a DNA crosslinker, BLM is a radiomimetic agent, MMS is an alkylating agent, and 4NQO is a UV-mimetic agent. The synergistic effect with BLM or MMS was described as not obvious.
Exposure Details
- SAR
- 3 W/kg
- Source/Device
- 1.8 GHz RFR along with chemical mutagens
Exposure Context
This study used 3 W/kg for SAR (device absorption):
- 7.5x above the Building Biology guideline of 0.4 W/kg
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
The aim of this investigation was to study the synergistic DNA damage effects in human lymphocytes induced by 1.8 GHz radiofrequency field radiation (RFR, SAR of 3 W/kg) with four chemical mutagens, i.e. mitomycin C (MMC, DNA crosslinker), bleomycin (BLM, radiomimetic agent), methyl methanesulfonate (MMS, alkylating agent), and 4-nitroquinoline-1-oxide (4NQO, UV-mimetic agent).
Three combinative exposure ways were used. Cells were exposed to RFR and chemical mutagens for 2 and...
The DNA damage of lymphocytes exposed to RFR and/or with chemical mutagens was detected at two incub...
The experimental results indicated 1.8 GHz RFR (SAR, 3 W/kg) for 2 h did not induce the human lymphocyte DNA damage effects in vitro, but could enhance the human lymphocyte DNA damage effects induced by MMC and 4NQO. The synergistic DNA damage effects of 1.8 GHz RFR with BLM or MMS were not obvious.
Show BibTeX
@article{wang_2005_studying_the_synergistic_damage_38,
author = {Baohong Wang and Jiliang H and Lifen J and Deqiang L and Wei Z and Jianlin L and Hongping D.},
title = {Studying the synergistic damage effects induced by 1.8 GHz radiofrequency field radiation (RFR) with four chemical mutagens on human lymphocyte DNA using comet assay in vitro.},
year = {2005},
url = {http://www.sciencedirect.com/science/article/pii/S0027510705002022},
}