8-oxoG DNA Glycosylase-1 Inhibition Sensitizes Neuro-2a Cells to Oxidative DNA Base Damage Induced by 900 MHz Radiofrequency Electromagnetic Radiation.
Wang X, Liu C, Ma Q, Feng W, Yang L, Lu Y, Zhou Z, Yu Z, Li W, Zhang L. · 2015
View Original AbstractExposure to 900 MHz RF-EMFs induced oxidative DNA base damage in Neuro-2a cells, but no DNA strand breakage or change in cell viability was observed.
Plain English Summary
Researchers exposed Neuro-2a cells to 900 MHz GSM signals at specific absorption rates of 0, 0.5, 1, or 2 W/kg for 24 hours, with or without silencing of the DNA repair enzyme OGG1. Without OGG1 silencing, 2 W/kg RF-EMFs induced oxidative DNA base damage, and with OGG1 silencing, damage was observed at exposures as low as 1 W/kg. Increases in oxidative DNA base damage were accompanied by similar increases in reactive oxygen species, but neither DNA strand breakage nor altered cell viability was observed.
Why This Matters
The study explored the in vitro putative genotoxicity of radiofrequency electromagnetic fields in Neuro-2a cells. DNA strand breakage was measured by the alkaline comet assay, and DNA base damage was measured by a modified comet assay using formamidopyrimidine DNA glycosylase. OGG1 silencing lowered the exposure level at which DNA base damage was detected, suggesting the enzyme may play a role in protecting DNA bases from RF-EMF-induced oxidative damage. The researchers described their conclusion as a hypothesis requiring further study.
Exposure Details
- SAR
- 0, 0.5, 1 or 2 W/kg
- Source/Device
- 900 MHz
- Exposure Duration
- 24 h
Where This Falls on the Concern Scale
Study Details
The purpose of this study was to explore the in vitro putative genotoxicity during exposure of Neuro-2a cells to radiofrequency electromagnetic fields (RF-EMFs) with or without silencing of 8-oxoG DNA glycosylase-1 (OGG1).
Neuro-2a cells treated with or without OGG1 siRNA were exposed to 900 MHz Global System for Mobile C...
Exposure to 900 MHz RF-EMFs with insufficient energy could induce oxidative DNA base damage in Neuro...
Even if further studies remain conducted we support the hypothesis that OGG1 is involved in the process of DNA base repair and may play a pivotal role in protecting DNA bases from RF-EMF induced oxidative damage.
Show BibTeX
@article{x_2015_8oxog_dna_glycosylase1_inhibition_577,
author = {Wang X and Liu C and Ma Q and Feng W and Yang L and Lu Y and Zhou Z and Yu Z and Li W and Zhang L.},
title = {8-oxoG DNA Glycosylase-1 Inhibition Sensitizes Neuro-2a Cells to Oxidative DNA Base Damage Induced by 900 MHz Radiofrequency Electromagnetic Radiation.},
year = {2015},
url = {https://www.karger.com/Article/Abstract/430233},
}