[Effect of microwave radiation on primary cultured Sertoli cells.]
Gao XF, Wang SM, Peng RY, Wang LF, Zuo HY, Gao YB, Dong Q, Dong B. · 2009
View Original AbstractMicrowave radiation at 100 mW/cm(2) may cause growth inhibition and increased apoptosis and death in primary cultured Sertoli cells, while 30 mW/cm(2) shifted the cell cycle without affecting apoptosis.
Plain English Summary
Researchers established a model of primary cultured Sertoli cells in vitro and exposed them to microwave radiation at average power densities of 0, 30, and 100 mW/cm(2) for five minutes. Cell cycle distribution shifted at exposure to 30 mW/cm(2) compared with the control groups, but the changes of rate of apoptosis and death and intracellular Ca2+ concentration showed no difference at 6 h after exposure. At 6 h after exposure to 100 mW/cm(2) microwave, there was a significant increase in intracellular Ca2+ concentration and rate of apoptosis and death, and the researchers identified the increase of intracellular Ca2+ concentration as one of the injury mechanisms.
Why This Matters
The cells were grown in vitro and exposed to microwave radiation at average power density 0, 30 and 100 mW/cm(2) for five minutes, then measured at 6 h after exposure. Measurements included cell cycle, apoptosis and death, and intracellular Ca2+ concentration, using Annexin V-PI double labeling and Fluo-3-AM labeling, flow cytometry combined with laser scanning confocal microscopy. At exposure to 30 mW/cm(2), Sertoli cells were obviously reduced in G0-G1 and G2-M phase and increased in S phase compared with the control groups, but apoptosis and calcium results did not differ significantly. At exposure to 100 mW/cm(2), there was a significant increase in the Sertoli cell counts of G0-G1 phase and a decrease in G2-M and S phase, alongside increased apoptosis, death, and intracellular Ca2+.
Exposure Details
- Power Density
- 0, 30 and 100 mW/cm²
Where This Falls on the Concern Scale
Study Details
To explore whether microwave radiation may cause injury of primary cultured Sertoli cells.
The model of primary cultured Sertoli cells in vitro was established, which was radiated by microwav...
The numbers of Sertoli cells were obviously reduced in G0-G1 and G2-M phase (62.57% +/- 3.22% and 8....
100 mW/cm(2) microwave radiation may cause growth inhibition and increase of apoptosis and death in the primary cultured Sertoli cells. The increase of intracellular Ca2+ concentration is one of the injury mechanisms.
Show BibTeX
@article{xf_2009_effect_of_microwave_radiation_989,
author = {Gao XF and Wang SM and Peng RY and Wang LF and Zuo HY and Gao YB and Dong Q and Dong B.},
title = {[Effect of microwave radiation on primary cultured Sertoli cells.]},
year = {2009},
url = {https://pubmed.ncbi.nlm.nih.gov/20137297/},
}