Exposure to extremely low-frequency electromagnetic fields modulates Na+ currents in rat cerebellar granule cells through increase of AA/PGE2 and EP receptor-mediated cAMP/PKA pathway.
He YL, Liu DD, Fang YJ, Zhan XQ, Yao JJ, Mei YA. · 2013
View Original AbstractExposing rat cerebellar granule cells to extremely low-frequency electromagnetic fields significantly increased sodium channel currents by 30–125% in a time- and intensity-dependent manner.
Plain English Summary
Researchers investigated the effects of extremely low-frequency electromagnetic fields (ELF-EMF) on sodium channel activity in rat cerebellar granule cells. Exposing the cells to ELF-EMF for 10–60 minutes significantly increased sodium currents by 30–125% in a time- and intensity-dependent manner. Increases in intracellular arachidonic acid, prostaglandin E2, and phosphorylated PKA levels were observed in the cells following ELF-EMF exposure. The data demonstrated for the first time that neuronal sodium currents are significantly increased by ELF-EMF exposure via a cPLA2, arachidonic acid, prostaglandin E2, EP receptors, and PKA signaling pathway.
Why This Matters
The steady-state activation curve of sodium channels was significantly shifted by 5.2 mV towards hyperpolarization by ELF-EMF stimulation, while the steady-state inactivation curve was not significantly affected. This effect on sodium currents in cerebellar granule cells was similar to that seen with intracellular application of arachidonic acid and prostaglandin E2. The amount of NaV 1.2 protein on the cell membrane increased after ELF-EMF exposure, though the total expression level of NaV 1.2 protein was not affected. When cyclooxygenase inhibitors and prostaglandin E2 receptor antagonists were applied, the ELF-EMF-induced increases in phosphorylated PKA and sodium currents were eliminated. ELF-EMF exposure significantly enhanced the activity of PLA2 in the cells but did not affect COX-1 or COX-2 activity.
Exposure Details
- Magnetic Field
- 1 and 0.4 mT
- Exposure Duration
- 10–60 min
Exposure Context
This study used 1 and 0.4 mT for magnetic fields:
- 20Kx above the Building Biology guideline of 0.2 mG
- 4Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
we investigated the effects of ELF-EMF on Nav activity in rat cerebellar granule cells (GCs).
Our results reveal that exposing cerebellar GCs to ELF-EMF for 10–60 min significantly increased Nav...
Together, these data demonstrate for the first time that neuronal INa is significantly increased by ELF-EMF exposure via a cPLA2 AA PGE2 EP receptors PKA signaling pathway.
Show BibTeX
@article{yl_2013_exposure_to_extremely_lowfrequency_258,
author = {He YL and Liu DD and Fang YJ and Zhan XQ and Yao JJ and Mei YA.},
title = {Exposure to extremely low-frequency electromagnetic fields modulates Na+ currents in rat cerebellar granule cells through increase of AA/PGE2 and EP receptor-mediated cAMP/PKA pathway.},
year = {2013},
url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0054376},
}