2.4 GHz Electromagnetic Field Influences the Response of the Circadian Oscillator in the Colorectal Cancer Cell Line DLD1 to miR-34a- Mediated Regulation
Authors not listed · 2022
Exposure to 2.4 GHz RF-EMF increased cry1 mRNA expression, weakened miR-34a's inhibition of per2 and abolished its inhibition of survivin, and eliminated miR-34a's effects on proliferation and migration in DLD1 cells.
Plain English Summary
Researchers exposed miR-34a-transfected DLD1 colorectal cancer cells to 2.4 GHz RF-EMF for 24 hours and evaluated effects using real-time PCR, scratch assay, and MTS test. Administration of miR-34a decreased expression of per2, bmal1, sirtuin1, and survivin and inhibited proliferation and migration of DLD1 cells. RF-EMF exposure increased cry1 mRNA expression, weakened miR-34a's inhibition of per2, abolished its inhibition of survivin, and eliminated miR-34a's effects on proliferation and migration. The authors concluded that RF-EMF strongly influenced miR-34a-mediated regulation of the peripheral circadian oscillator in DLD1 cells.
Why This Matters
Radiofrequency electromagnetic fields exert pleiotropic effects on biological processes including circadian rhythms. miR-34a is a small non-coding RNA whose expression is modulated by RF-EMF and can regulate clock gene expression. The interference between RF-EMF and miR-34a-mediated regulation of the circadian oscillator had not been elucidated before this study.
Exposure Information
Specific exposure levels were not quantified in this study.
Show BibTeX
@article{24_ghz_electromagnetic_field_influences_the_response_of_the_circadian_oscillator_in_the_colorectal_cancer_cell_line_dld1_to_mir_34a_mediated_regulation_ce2954,
author = {Unknown},
title = {2.4 GHz Electromagnetic Field Influences the Response of the Circadian Oscillator in the Colorectal Cancer Cell Line DLD1 to miR-34a- Mediated Regulation},
year = {2022},
doi = {10.3390/ijms232113210},
}