Comparison of effects of high- and low-frequency electromagnetic fields on proliferation and differentiation of neural stem cells
Bai W, Li M, Xu W, Zhang M · 2021
Both low- and high-frequency EMFs promoted neural stem cell proliferation in vitro, and low-frequency EMF accelerated neuronal differentiation.
Plain English Summary
Researchers obtained neural stem cells from rat hippocampus and exposed them to a low-frequency field (5 m T, 50 Hz, 30 min daily) or a high-frequency field (maximum magnetic induction 2.5 T, 40 % MO, 50 Hz, 10 min daily) with a no-field control. Low-frequency EMF also yielded the highest proportion of Tuj-1-positive cells and Tuj-1 mRNA expression, while GFAP-positive cells and GFAP mRNA did not differ among groups. The authors concluded that both EMF types promote proliferation in vitro and that low-frequency EMF accelerates differentiation into neurons.
Why This Matters
The study aimed to compare how high- and low-frequency electromagnetic fields affect neural stem cell proliferation and differentiation. Cell viability and quantity were assessed at three days using a CCK-8 assay and a cell counting plate. Immunofluorescence staining and quantitative PCR were used to measure the percentages of Tuj-1- and GFAP-positive cells and their mRNA levels.
Show BibTeX
@article{bai_w_li_m_xu_w_zhang_m_ce3965,
author = {Bai W and Li M and Xu W and Zhang M},
title = {Comparison of effects of high- and low-frequency electromagnetic fields on proliferation and differentiation of neural stem cells},
year = {2021},
}