Exposure to Pulsed Electromagnetic Fields Improves the Developmental Competence and Quality of Somatic Cell Nuclear Transfer Buffalo ( Bubalus bubalis) Embryos Produced Using Fibroblast Cells and Alters Their Epigenetic Status and Gene Expression
Authors not listed · 2021
Pulsed electromagnetic field treatment of buffalo SCNT embryos increased blastocyst formation, lowered apoptosis, and altered gene expression.
Plain English Summary
PEMF treatment of cumulus cells at 30 µT for three hours increased cell viability, proliferation, and expression of OCT4, NANOG, SOX2, P53, CCNB1, and GPX while decreasing DNMT1, DNMT3a, GSK3b, and BAX, and PEMF treatment of SCNT embryos at the start of culture raised the blastocyst rate from 42.8% to 51.4% and lowered the apoptotic index to IVF levels without changing total cell number or inner cell mass to trophectoderm ratio. In blastocysts, the treatment up-regulated NANOG, SOX2, CDX2, GLUT1, P53, and BCL2 and down-regulated BAX, CASPASE3, GSK3b, and HSP70 while leaving OCT4, DNMT1, DNMT3a, DNMT3b, HDAC1, and CCNB1 unchanged, and it increased global H3K27me3 without affecting H3K18ac.
Show BibTeX
@article{exposure_to_pulsed_electromagnetic_fields_improves_the_developmental_competence_and_quality_of_somatic_cell_nuclear_transfer_buffalo_bubalus_bubalis_embryos_produced_using_fibroblast_cells_and_alters_ce4210,
author = {Unknown},
title = {Exposure to Pulsed Electromagnetic Fields Improves the Developmental Competence and Quality of Somatic Cell Nuclear Transfer Buffalo ( Bubalus bubalis) Embryos Produced Using Fibroblast Cells and Alters Their Epigenetic Status and Gene Expression},
year = {2021},
doi = {10.1089/cell.2021.0028},
}