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Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field.

No Effects Found

Nakayama M, Nakamura A, Hondou T, Miyata H · 2016

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Magnetic field exposure amplified DNA damage in immune cells already stressed by bacterial toxins, suggesting EMFs may worsen harm during illness.

Plain English Summary

Summary written for general audiences

Researchers exposed immune cells called macrophages to 50-Hz magnetic fields (the same frequency as power lines) for 24 hours to see if it would damage their DNA. They found that magnetic field exposure alone caused no harm, but when cells were first activated by bacterial toxins, the magnetic field exposure increased DNA damage and reduced cell survival.

Study Details

To determine whether this hypothesis holds in macrophage RAW264 cells, we measured DNA single-strand breaks (SSB), cell viability, and nitric oxide (NO) production in cells with or without exposure to 0.5-mT, 50-Hz magnetic fields for 24 h and with or without simultaneous stimulation via the bacterial endotoxin, lipopolysaccharide (LPS).

Macrophages stimulated with 10 ng/ml LPS for 1 h were exposed to or not exposed to a magnetic field ...

The 50-Hz magnetic field enhanced DNA SSB and decreased cell viability only in the LPS-stimulated ma...

Co-stimulation of the cell with LPS and a 50-Hz magnetic field promoted SSB and lowered cell viability, but these were not mediated by LPS-induced NO production.

Cite This Study
Nakayama M, Nakamura A, Hondou T, Miyata H (2016). Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field. Int J Radiat Biol. 92(10):583-589, 2016.
Show BibTeX
@article{m_2016_evaluation_of_cell_viability_2857,
  author = {Nakayama M and Nakamura A and Hondou T and Miyata H},
  title = {Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field. },
  year = {2016},
  doi = {10.1080/09553002.2016.1206224},
  url = {https://www.tandfonline.com/doi/abs/10.1080/09553002.2016.1206224},
}

Quick Questions About This Study

Researchers exposed immune cells called macrophages to 50-Hz magnetic fields (the same frequency as power lines) for 24 hours to see if it would damage their DNA. They found that magnetic field exposure alone caused no harm, but when cells were first activated by bacterial toxins, the magnetic field exposure increased DNA damage and reduced cell survival.