Microarray profiling of LncRNA expression in the testis of pubertal mice following morning and evening exposure to 1800 MHz radiofrequency fields
Qin F, Cao H, Feng C, Zhu T, Zhu B, Zhang J, Tong J, Pei H · 2021
The findings suggest that 1800 MHz RF induced testicular toxicity and lncRNA expression changes that differed between morning and evening exposure in pubertal mice.
Plain English Summary
Researchers exposed four-week-old mice to 1800 MHz RF at 0.50 W/kg SAR in the morning and evening each day for three weeks. They measured pathological changes and functional indices in the testes. RF exposure reduced testicular weight, daily sperm production, and testosterone secretion. Morning exposure was associated with 615 differentially expressed lncRNAs, while evening exposure was associated with 183. Morning RF exposure lncRNAs were correlated with pathways including Fanconi syndrome, metabolic processes, cell cycle, DNA damage, and DNA replication.
Why This Matters
The study used lncRNA microarray and GO/KEGG pathway analyses to profile lncRNA expression and predict functions. Cis and trans regulation of lncRNAs were examined and an interaction network was built with Cytoscape. Six lncRNAs common to both morning and evening exposure groups were validated by qRT-PCR. Trans-regulation analyses linked differentially expressed lncRNAs to transcription factors such as TCFAP4, NFkB, HINFP, TFDP2, FoxN1, and PAX5. These transcription factors are known to modulate testis development, cell cycle progression, and spermatogenesis.
Show BibTeX
@article{qin_f_cao_h_feng_c_zhu_t_zhu_b_zhang_j_tong_j_pei_h_ce2977,
author = {Qin F and Cao H and Feng C and Zhu T and Zhu B and Zhang J and Tong J and Pei H},
title = {Microarray profiling of LncRNA expression in the testis of pubertal mice following morning and evening exposure to 1800 MHz radiofrequency fields},
year = {2021},
}