Reduction of Phosphorylated Synapsin I (Ser-553) Leads to Spatial Memory Impairment by Attenuating GABA Release after Microwave Exposure in Wistar Rats
Qiao S, Peng R, Yan H, Gao Y, Wang C, Wang S, Zou Y, Xu X, Zhao L, Dong J, Su Z, Feng X, Wang L, Hu X · 2014
View Original AbstractMicrowave exposure in rats reduced spatial memory performance and attenuated GABA release, with phosphorylated synapsin I (ser-553) found to play a key role in this effect.
Plain English Summary
Researchers exposed Wistar rats, rat hippocampal synaptosomes, and PC12 cells to microwave radiation for 5 minutes at a mean power density of 30 mW/cm2. Spatial learning and memory in rats was assessed using the Morris Water Maze navigation task, and there was a decrease in spatial memory performance after microwave exposure. The expression of phosphorylated synapsin I (ser-553) was decreased at 3 days post-exposure and elevated at later time points, while GABA release from synaptosomes was attenuated. Both microwave exposure and synapsin I silencing reduced GABA release, and the greatest reduction was found when both were combined, indicating a synergetic effect.
Why This Matters
The study investigated what role phosphorylated synapsin I (p-Syn I) might play in the memory and neurotransmitter effects seen after microwave exposure. Sham group rats, synaptosomes, and cells were otherwise identically treated and acted as controls for all of the post-exposure analyses. Vesicular GABA transporter (VGAT) was significantly elevated after exposure, and both p-Syn I (ser-553) and VGAT were enriched in small clear synaptic vesicles that abnormally assembled in the presynaptic terminal after exposure. In PC12 cell experiments, the expression of p-Syn I (ser-553) and GABA release were both attenuated at 6 hours after exposure. Researchers concluded that p-Syn I (ser-553) played a key role in the impaired GABA release and cognitive dysfunction induced by microwave exposure.
Exposure Details
- Power Density
- 30 mW/cm²
- Exposure Duration
- 5 min
Exposure Context
This study used 30 mW/cm² for radio frequency:
- 3,000Mx above the Building Biology guideline of 0.1 μW/m²
- 50Mx above the BioInitiative Report recommendation of 0.0006 μW/cm²
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
This study investigated the mechanism of this effect by exploring the potential role of phosphorylated synapsin I (p-Syn I).
Wistar rats, rat hippocampal synaptosomes, and differentiated (neuronal) PC12 cells were exposed to ...
In the rat experiments, there was a decrease in spatial memory performance after microwave exposure....
p-Syn I (ser-553) was found to play a key role in the impaired GABA release and cognitive dysfunction that was induced by microwave exposure.
Show BibTeX
@article{s_2014_reduction_of_phosphorylated_synapsin_170,
author = {Qiao S and Peng R and Yan H and Gao Y and Wang C and Wang S and Zou Y and Xu X and Zhao L and Dong J and Su Z and Feng X and Wang L and Hu X},
title = {Reduction of Phosphorylated Synapsin I (Ser-553) Leads to Spatial Memory Impairment by Attenuating GABA Release after Microwave Exposure in Wistar Rats},
year = {2014},
url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095503},
}