Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells.
Nikolova T, Czyz J, Rolletschek A, Blyszczuk P, Fuchs J, Jovtchev G, Schuderer J, Kuster N, Wobus AM · 2005
View Original AbstractELF-EMF and RF-EMF exposure transiently altered transcript levels of apoptosis-related and cell cycle genes in neural progenitor cells without changing cell physiology.
Plain English Summary
ELF-EMF significantly affected transcript levels of bcl-2, bax, and GADD45 genes but did not affect neural-specific gene mRNA levels. RF-EMF down-regulated neural-specific Nurr1 and up-regulated bax and GADD45 mRNA levels. Short-term RF-EMF exposure for 6 hours caused a transient increase in DNA double-strand breaks, while 48-hour exposure did not. No effects were observed on mitochondrial function, nuclear apoptosis, cell proliferation, or chromosomal alterations.
Why This Matters
The study used mouse embryonic stem cells as a model to investigate electromagnetic field effects. Cells were analyzed for transcript levels of cell cycle regulatory, apoptosis-related, and neural-specific genes and proteins, as well as proliferation, apoptosis, and cytogenetic effects.
Exposure Details
- Magnetic Field
- 2 mT
- SAR
- 1.5 W/kg
- Source/Device
- 50 Hz (ELF-EMF) and 1.71 GHz (RF-EMF)
- Exposure Duration
- 6 h and 48 h
Exposure Context
This study used 2 mT for magnetic fields:
- 100Kx above the Building Biology guideline of 0.2 mG
- 20Kx above the BioInitiative Report recommendation of 1 mG
This study used 1.5 W/kg for SAR (device absorption):
- 3.8x above the Building Biology guideline of 0.4 W/kg
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
Mouse embryonic stem (ES) cells were used as an experimental model to study the effects of electromagnetic fields (EMF).
ES-derived nestin-positive neural progenitor cells were exposed to extremely low frequency EMF simul...
Quantitative RT-PCR analysis revealed that ELF-EMF exposure to ES-derived neural cells significantly...
We may conclude that EMF exposure of ES-derived neural progenitor cells transiently affects the transcript level of genes related to apoptosis and cell cycle control. However, these responses are not associated with detectable changes of cell physiology, suggesting compensatory mechanisms at the translational and posttranslational level.
Show BibTeX
@article{t_2005_electromagnetic_fields_affect_transcript_1225,
author = {Nikolova T and Czyz J and Rolletschek A and Blyszczuk P and Fuchs J and Jovtchev G and Schuderer J and Kuster N and Wobus AM},
title = {Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells.},
year = {2005},
doi = {10.1096/fj.04-3549fje},
url = {https://faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.04-3549fje},
}