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Reproductive Health

The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells

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Authors not listed · 2021

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Sertoli-derived exosomes ameliorated electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.

Plain English Summary

Summary written for general audiences

Researchers isolated spermatogonial stem cells and Sertoli cells from neonate mouse testes. The stem cells were exposed to 50 Hz electromagnetic fields at 2.5 mT for one hour daily over five days and then treated with exosomes from pre-pubertal Sertoli cells. Electromagnetic field exposure increased reactive oxygen species, reduced catalase expression, cell viability, and colonization, and increased apoptosis and Caspase-3 expression while decreasing GFRα1 expression. Sertoli-derived exosomes significantly improved these adverse effects by regulating oxidative stress.

Why This Matters

The exosomes were characterized and their uptake by stem cells was confirmed using PKH26 labeling. The researchers assessed cell viability, colonization efficiency, reactive oxygen species balance, cell cycle arrest, and apoptosis induction. The authors suggest that Sertoli-derived exosomes could serve as a novel therapeutic agent to restore the spermatogonial stem cell microenvironment and spermatogenesis after electromagnetic field exposure.

Exposure Information

A logarithmic frequency spectrum from 10 Hz to 100 GHz showing where this study's 50 Hz exposure sits relative to common EMF sources.Where This Frequency Sits on the EMF SpectrumELFVLFLF / MFHF / VHFUHFSHFmm10 Hz100 GHzThis study: 50 HzCell phones~1 GHzWiFi2.4 GHz5G mm28 GHzLogarithmic scale

Specific exposure levels were not quantified in this study.

Cite This Study
Unknown (2021). The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.
Show BibTeX
@article{the_guardians_of_germ_cells_sertoli_derived_exosomes_against_electromagnetic_field_induced_oxidative_stress_in_mouse_spermatogonial_stem_cells_ce3942,
  author = {Unknown},
  title = {The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells},
  year = {2021},
  doi = {10.1016/j.theriogenology.2021.08.001},
  
}
DOI: 10.1016/j.theriogenology.2021.08.001PubMed: 34371438

Quick Questions About This Study

Mouse spermatogonial stem cells were exposed to 50 Hz electromagnetic fields at 2.5 mT for one hour per day over five days.
Exposure increased reactive oxygen species, reduced catalase antioxidant expression, cell viability, and colonization, and increased apoptosis and Caspase-3 expression while decreasing GFRα1 expression.
Administration of Sertoli-derived exosomes significantly improved the adverse changes by regulating oxidative stress.
Spermatogonial stem cells were confirmed by Oct4 and Plzf immunocytochemistry, while Sertoli cells were identified by Sox9 and vimentin expression using immunocytochemistry and quantitative real-time PCR.