Osteogenic differentiation of amniotic epithelial cells: synergism of pulsed electromagnetic field and biochemical stimuli
Wang Q, Wu W, Han X, Zheng A, Lei S, Wu J, Chen H, He C, Luo F, Liu X · 2014
A combination of pulsed electromagnetic field and osteo-induction medium synergistically enhanced osteogenic differentiation of amniotic epithelial cells.
Plain English Summary
Researchers isolated amniotic epithelial cells from human placenta and exposed them to pulsed electromagnetic field and/or osteo-induction medium for 21 days. PEMF alone or osteo-induction medium alone induced osteogenic differentiation as shown by expression of alkaline phosphatase and osteocalcin. The combination of PEMF and osteo-induction medium had synergistic effects on osteogenic differentiation. Gene expression of BMP-2, Runx2, β-catenin, Nrf2, Keap1, and integrinβ1 was up-regulated during osteogenic differentiation.
Why This Matters
The researchers investigated whether PEMF could modulate osteogenic differentiation of amniotic epithelial cells, a potential stem cell source for cell therapy, because this was unknown. They also examined signal transduction pathways involved in osteogenesis. The authors suggest that the combined treatment might be a novel approach in bone regenerative medicine.
Show BibTeX
@article{wang_q_wu_w_han_x_zheng_a_lei_s_wu_j_chen_h_he_c_luo_f_liu_x_ce4253,
author = {Wang Q and Wu W and Han X and Zheng A and Lei S and Wu J and Chen H and He C and Luo F and Liu X},
title = {Osteogenic differentiation of amniotic epithelial cells: synergism of pulsed electromagnetic field and biochemical stimuli},
year = {2014},
}