Extremely low-frequency electromagnetic field exposure alters DNA methylation levels in the endometrium of pigs during the peri-implantation period
Wydorski PJ, Kozlowska W, Drzewiecka, EM, Zmijewska A, Franczak A · 2023
Porcine endometrial tissue exposed to 50 Hz ELF-EMF for 2 hours showed increased methylation of EGR2, ID2, and PTGER4, decreased methylation of IL1RAP and NOS3, and no change in HSD17B2, MRAP2, SERPINE1, VDR, and ZFP57.
Plain English Summary
Researchers collected porcine endometrial slices during the peri-implantation period and exposed them to 50 Hz ELF-EMF for 2 hours in vitro. They measured DNA methylation in the promoter regions of EGR2, HSD17B2, ID2, IL1RAP, MRAP2, NOS3, PTGER4, SERPINE1, VDR and ZFP57 using qMS-PCR. Exposure increased methylation of EGR2, ID2, and PTGER4, decreased methylation of IL1RAP and NOS3, and did not change methylation of HSD17B2, MRAP2, SERPINE1, VDR, and ZFP57.
Why This Matters
The researchers hypothesised that short-term ELF-EMF treatment alters the DNA methylation level of genes in the endometrium. They concluded that ELF-EMF may alter the level of DNA methylation in the endometrium during the peri-implantation period. Changes in the DNA methylation induced by ELF-EMF may affect the transcriptomic profile of the endometrium and disturb physiological processes accompanying implantation and embryo development.
Exposure Information
Specific exposure levels were not quantified in this study.
Show BibTeX
@article{wydorski_pj_kozlowska_w_drzewiecka_em_zmijewska_a_franczak_a_ce4261,
author = {Wydorski PJ and Kozlowska W and Drzewiecka and EM and Zmijewska A and Franczak A},
title = {Extremely low-frequency electromagnetic field exposure alters DNA methylation levels in the endometrium of pigs during the peri-implantation period},
year = {2023},
doi = {10.1071/RD22266},
}