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NADPH oxidase-produced superoxide mediated a 50-Hz magnetic field-induced epidermal growth factor receptor clustering

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Feng B, Dai A, Chen L, Qiu L, Fu Y, Sun W. · 2016

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Nox-produced superoxide mediated a 50-Hz magnetic field-induced EGFR clustering in FL cells.

Plain English Summary

Summary written for general audiences

Researchers exposed human amniotic epithelial (FL) cells to a 50-Hz magnetic field to investigate the molecular mechanisms behind magnetic field-induced epidermal growth factor receptor clustering. Exposing FL cells to the magnetic field at intensities higher than 0.2 mT for 15 minutes enhanced total reactive oxygen species production. Blocking the NADPH oxidase enzyme with the inhibitor DPI suppressed magnetic field-induced cytoplasmic superoxide production and subsequent EGFR clustering, while mitochondrial superoxide production was not affected.

Why This Matters

The study built on earlier work that had already shown a 50-Hz magnetic field could induce EGFR clustering, and it set out to identify the molecular steps involved. Enhanced cytoplasmic superoxide production was observed after exposing cells to the magnetic field at 0.4 mT for 5, 15, or 30 minutes. Researchers measured total ROS, cytoplasmic superoxide, and mitochondrial superoxide using the probes DCFH-DA, DHE, and MitoSOX, respectively, and examined EGFR clustering by confocal microscopy.

Exposure Details

Magnetic Field
0.4 mT
Source/Device
50 Hz
Exposure Duration
5, 15, 30 or 60 minutes

Exposure Context

This study used 0.4 mT for magnetic fields:

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Exposure Level in ContextStudy Exposure Level in ContextThis study: 0.4 mTExtreme Concern - 5 mGFCC Limit - 2,000 mGEffects observed in the Extreme Concern range (Building Biology)This level is 2 times the FCC limit
A logarithmic frequency spectrum from 10 Hz to 100 GHz showing where this study's 50 Hz exposure sits relative to common EMF sources.Where This Frequency Sits on the EMF SpectrumELFVLFLF / MFHF / VHFUHFSHFmm10 Hz100 GHzThis study: 50 HzCell phones~1 GHzWiFi2.4 GHz5G mm28 GHzLogarithmic scale

Study Details

A 50-Hz magnetic field (MF) was found to induce epidermal growth factor receptor (EGFR) clustering in our previous study. The aim of this work was to investigate the molecular mechanisms that mediated MF-induced EGFR clustering.

Human amniotic epithelial (FL) cells were exposed to a 50-Hz MF. Total reactive oxygen species (ROS)...

Results showed that exposing FL cells to MF at intensity higher than 0.2 mT for 15 min enhanced tota...

Nox-produced superoxide mediated a 50-Hz magnetic field-induced EGFR clustering.

Cite This Study
Feng B, Dai A, Chen L, Qiu L, Fu Y, Sun W. (2016). NADPH oxidase-produced superoxide mediated a 50-Hz magnetic field-induced epidermal growth factor receptor clustering Int J Radiat Biol. 92:596-602, 2016b.
Show BibTeX
@article{b_2016_nadph_oxidaseproduced_superoxide_mediated_360,
  author = {Feng B and Dai A and Chen L and Qiu L and Fu Y and Sun W.},
  title = {NADPH oxidase-produced superoxide mediated a 50-Hz magnetic field-induced epidermal growth factor receptor clustering},
  year = {2016},
  doi = {10.1080/09553002.2016.1206227},
  url = {https://www.tandfonline.com/doi/abs/10.1080/09553002.2016.1206227},
}
DOI: 10.1080/09553002.2016.1206227

Cited By (7 papers)

Quick Questions About This Study

The experiments used human amniotic epithelial cells, referred to as FL cells.
Total ROS production was enhanced when FL cells were exposed to the magnetic field at intensities higher than 0.2 mT for 15 minutes.
Nox-produced superoxide mediated the 50-Hz magnetic field-induced EGFR clustering, and blocking it with DPI inhibited cytoplasmic superoxide production and subsequent EGFR clustering.
Mitochondrial superoxide production was not affected by pretreatment with the NADPH oxidase inhibitor DPI.
EGFR clustering was analyzed using confocal microscopy after indirect immunofluorescence staining.