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Exposure to electromagnetic field attenuates oxygen-glucose deprivation-induced microglial cell death by reducing intracellular Ca2+ and ROS.

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Duong CN, Kim JY · 2016

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Exposure to 50 Hz/1 mT EMF reduced cell death and lowered intracellular calcium and ROS levels in human microglial cells under oxygen-glucose deprivation.

Plain English Summary

Summary written for general audiences

Researchers studied whether EMF exposure could protect a human microglial cell line, HMO6, from cell death caused by oxygen-glucose deprivation (OGD), a model of ischemic injury. HMO6 cells were cultured for 4 hours under OGD with or without exposure to EMF at different combinations of frequencies and intensities, and cell survival, intracellular calcium, and ROS levels were measured. Among the combinations tested, 50 Hz/1 mT EMF was the most potent at reducing OGD-induced cell death and lowering intracellular calcium and ROS levels. A significant but less potent protective effect was also found at 10 Hz/1 mT, while no protective effect was found at other combinations.

Why This Matters

OGD caused significant HMO6 cell death as well as elevation of intracellular calcium and ROS levels. The researchers also examined which enzymes contributed to OGD-induced ROS production and cell death. A xanthine oxidase inhibitor reversed OGD-induced ROS production and cell death, while NADPH oxidase and mitochondrial respiration chain complex II inhibitors did not affect cell death. The researchers concluded that xanthine oxidase is one of the main mediators involved in OGD-induced HMO6 cell death. The authors suggested that non-invasive treatment of EMF radiation may be clinically useful to attenuate hypoxic-ischemic brain injury.

Exposure Details

Magnetic Field
1 mT
Source/Device
10, 50, or 100 Hz
Exposure Duration
4 hours

Exposure Context

This study used 1 mT for magnetic fields:

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Exposure Level in ContextStudy Exposure Level in ContextThis study: 1 mTExtreme Concern - 5 mGFCC Limit - 2,000 mGEffects observed in the Extreme Concern range (Building Biology)This level is 5 times the FCC limit
A logarithmic frequency spectrum from 10 Hz to 100 GHz showing where this study's 100 Hz exposure sits relative to common EMF sources.Where This Frequency Sits on the EMF SpectrumELFVLFLF / MFHF / VHFUHFSHFmm10 Hz100 GHzThis study: 100 HzCell phones~1 GHzWiFi2.4 GHz5G mm28 GHzLogarithmic scale

Study Details

The aim of this research was to demonstrate the protective effects of electromagnetic field (EMF) exposure on the human microglial cell line, HMO6, against ischemic cell death induced by in vitro oxygen-glucose deprivation (OGD).

HMO6 cells were cultured for 4 h under OGD with or without exposure to EMF with different combinatio...

OGD caused significant HMO6 cell death as well as elevation of intracellular Ca2+ and ROS levels. Am...

50 Hz/1 mT EMF protects human microglial cells from OGD-induced cell death by interfering with OGD-induced elevation of intracellular Ca2+ and ROS levels, and xanthine oxidase is one of the main mediators involved in OGD-induced HMO6 cell death. Non-invasive treatment of EMF radiation may be clinically useful to attenuate hypoxic-ischemic brain injury.

Cite This Study
Duong CN, Kim JY (2016). Exposure to electromagnetic field attenuates oxygen-glucose deprivation-induced microglial cell death by reducing intracellular Ca2+ and ROS. Int J Radiat Biol. 92(4):195-201, 2016.
Show BibTeX
@article{cn_2016_exposure_to_electromagnetic_field_351,
  author = {Duong CN and Kim JY},
  title = {Exposure to electromagnetic field attenuates oxygen-glucose deprivation-induced microglial cell death by reducing intracellular Ca2+ and ROS.},
  year = {2016},
  doi = {10.3109/09553002.2016.1136851},
  url = {https://www.tandfonline.com/doi/abs/10.3109/09553002.2016.1136851},
}
DOI: 10.3109/09553002.2016.1136851

Cited By (32 papers)

Quick Questions About This Study

The study used the human microglial cell line HMO6.
50 Hz/1 mT EMF was the most potent at reducing OGD-induced cell death and lowering intracellular calcium and ROS levels.
A significant but less potent protective effect was found at 10 Hz/1 mT, whereas no protective effect was found at other combinations of EMF.
OGD caused significant HMO6 cell death as well as elevation of intracellular calcium and ROS levels.
A xanthine oxidase inhibitor reversed OGD-induced ROS production and cell death, pointing to xanthine oxidase as one of the main mediators involved in OGD-induced HMO6 cell death.