Effect of Low-Frequency, Low-Energy Pulsed Electromagnetic Fields in Neuronal and Microglial Cells Injured with Amyloid- Beta
Authors not listed · 2024
PEMFs defended SH-SY5Y cells against Aβ peptide- or H2O2-induced oxidative stress, mitochondrial damage, and cell death, and reverted the reduction in mitochondrial potential, oxidative damage, and cell death in microglia activated by LPS or Aβ peptide.
Plain English Summary
Researchers stimulated SH-SY5Y neuronal cells with hydrogen peroxide or amyloid-β peptide and activated N9 microglial cells with lipopolysaccharide or amyloid-β peptide. Cells were exposed to pulsed electromagnetic fields with a pulse duration of 1.3 ms and a frequency of 75 Hz. The fields defended SH-SY5Y cells against oxidative stress, mitochondrial damage, and cell death induced by amyloid-β or hydrogen peroxide. In microglia activated by lipopolysaccharide or amyloid-β, the fields reverted the reduction in mitochondrial potential, oxidative damage, and cell death.
Why This Matters
The study aimed to investigate the effects of PEMF exposure on oxidative stress and cell death in in vitro-injured cellular models of neurons and microglia. Alzheimer’s disease is a neurodegenerative pathology covering about 70% of all cases of dementia and is associated with neuroinflammation and neuronal cell death. Data in the literature suggest several neuroprotective effects of low-frequency, low-energy pulsed electromagnetic fields on biological systems, and clinical studies report that PEMF stimulation is safe and well tolerated. The researchers measured reactive oxygen production, mitochondrial integrity, and cell death using H2DCFDA, JC-1, fluorescence, and MTS assays. Overall, the findings imply that PEMFs influence the redox state of the cells by significantly boosting antioxidant levels in both injured microglia and neuronal in vitro cells mimicking in vitro AD.
Exposure Information
Specific exposure levels were not quantified in this study.
Show BibTeX
@article{effect_of_low_frequency_low_energy_pulsed_electromagnetic_fields_in_neuronal_and_microglial_cells_injured_with_amyloid_beta_ce3937,
author = {Unknown},
title = {Effect of Low-Frequency, Low-Energy Pulsed Electromagnetic Fields in Neuronal and Microglial Cells Injured with Amyloid- Beta},
year = {2024},
doi = {10.3390/ijms252312847},
}