RKIP Regulates Neural Cell Apoptosis Induced by Exposure to Microwave Radiation Partly Through the MEK/ERK/CREB Pathway
Zuo H, Lin T, Wang D, Peng R, Wang S, Gao Y, Xu X, Zhao L, Wang S, Su Z. · 2015
View Original AbstractRKIP plays an important role in neural cell apoptosis induced by microwave radiation, partly through the MEK/ERK/CREB pathway.
Plain English Summary
Researchers exposed differentiated PC12 cells to continuous microwave radiation at 2.856 GHz for 5 minutes with an average power density of 30 mW/cm2. After exposure, RKIP was downregulated while the MEK/ERK/CREB signaling pathway was activated excessively. The ratio of Bcl-2/Bax decreased, caspase-3 activity increased, and apoptotic DNA fragmentation increased. The researchers suggested that RKIP may act as a key regulator of neuronal damage caused by microwave radiation.
Why This Matters
The study investigated whether a protein called RKIP is important for neural cell apoptosis induced by microwave exposure and explored the role of the MEK/ERK/CREB pathway regulated by RKIP. RKIP sense and anti-sense recombinant plasmids were constructed and transfected into PC12 cells to alter RKIP levels. When RKIP was overexpressed, it significantly inhibited the phosphorylation of MEK, ERK, and CREB, while reducing RKIP had the reverse effect. A compound called U0126 was found to antagonize the changes caused by RKIP downregulation after exposure to radiation. Cell apoptosis was detected using TUNEL staining and a caspase-3 activity assay.
Exposure Details
- Power Density
- 30 mW/cm²
- Source/Device
- 2.856 GHz
- Exposure Duration
- 5 min
Exposure Context
This study used 30 mW/cm² for radio frequency:
- 3,000Mx above the Building Biology guideline of 0.1 μW/m²
- 50Mx above the BioInitiative Report recommendation of 0.0006 μW/cm²
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
In the present study, we investigated whether Raf-1 kinase inhibitory protein (RKIP) is important for neural cell apoptosis induced by microwave exposure and explored the role of MEK/ERK/CREB pathway regulated by RKIP in the apoptosis.
Differentiated PC12 cells were exposed to continuous microwave radiation at 2.856 GHz for 5 min with...
The results showed that RKIP was downregulated after microwave exposure while the MEK/ERK/CREB signa...
In conclusion, RKIP plays an important role in the neural cell apoptosis induced by microwave radiation, and the regulation of cell apoptosis by RKIP is partly through the MEK/ERK/CREB pathway. This suggests that RKIP may act as a key regulator of neuronal damage caused by microwave radiation.
Show BibTeX
@article{h_2015_rkip_regulates_neural_cell_213,
author = {Zuo H and Lin T and Wang D and Peng R and Wang S and Gao Y and Xu X and Zhao L and Wang S and Su Z. },
title = {RKIP Regulates Neural Cell Apoptosis Induced by Exposure to Microwave Radiation Partly Through the MEK/ERK/CREB Pathway},
year = {2015},
doi = {10.1007/s12035-014-8831-5},
url = {https://link.springer.com/article/10.1007/s12035-014-8831-5},
}