Pulsed electromagnetic field stimulates cellular proliferation in human intervertebral disc cells.
Lee HM, Kwon UH, Kim H, Kim HJ, Kim B, Park JO, Moon ES, Moon SH. · 2010
View Original AbstractPulsed EMF stimulated DNA synthesis in human intervertebral disc cells, while proteoglycan synthesis and collagen gene expression were unchanged.
Plain English Summary
Researchers cultured human intervertebral disc (IVD) cells in alginate beads and exposed them to an electromagnetic field at 1.8 millitesla magnetic flux density and 60 Hz. Cellular proliferation was observed in the EMF group (p < 0.05), and no cytotoxicity was found. There was no significant change in the expression of aggrecan, type I, and type II collagen mRNA in the EMF group compared to the control group. DNA synthesis was partially mediated by nitric oxide and prostaglandin E2.
Why This Matters
The cells were cultured three-dimensionally in alginate beads. Cultures with NMMA (blocker of nitric oxide) or ASA (blocker of prostaglandin E2) exposed to EMF demonstrated decreased DNA synthesis compared to control cultures without NMMA or ASA (p < 0.05). There was no difference in newly synthesized proteoglycan normalized by DNA synthesis between the EMF group and the control. The researchers suggested that EMF can be utilized to stimulate proliferation of IVD cells, which may provide efficient cell amplification in cell therapy to degenerative disc disease.
Exposure Details
- Magnetic Field
- 1.8 mT
- Source/Device
- 60 Hz
- Exposure Duration
- 72-hour
Exposure Context
This study used 1.8 mT for magnetic fields:
- 90Kx above the Building Biology guideline of 0.2 mG
- 18Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
The purpose of this study is to investigate the mechanism of cellular proliferation of electromagnetic field (EMF) on human intervertebral disc (IVD) cells.
Human IVD cells were cultured three-dimensionally in alginate beads. EMF was exposed to IVD cells wi...
There was no cytotoxicity in IVD cells with the EMF group in MTT assay. Cellular proliferation was o...
EMF stimulated DNA synthesis in human IVD cells while no significant effect on proteoglycan synthesis and chondrogenic phenotype expressions. DNA synthesis was partially mediated by nitric oxide and prostaglandin E2. EMF can be utilized to stimulate proliferation of IVD cells, which may provide efficient cell amplification in cell therapy to degenerative disc disease.
Show BibTeX
@article{hm_2010_pulsed_electromagnetic_field_stimulates_407,
author = {Lee HM and Kwon UH and Kim H and Kim HJ and Kim B and Park JO and Moon ES and Moon SH.},
title = {Pulsed electromagnetic field stimulates cellular proliferation in human intervertebral disc cells.},
year = {2010},
url = {https://synapse.koreamed.org/articles/1030733},
}