Neuronal Cellular Responses to Extremely Low Frequency Electromagnetic Field Exposure: Implications Regarding Oxidative Stress and Neurodegeneration.
Reale M, Kamal MA, Patruno A, Costantini E, D'Angelo C, Pesce M, Greig NH · 2014
View Original AbstractELF-EMF exposure elevated markers of oxidative stress in human neuronal cells, with compensatory antioxidant responses that were less effective when combined with H2O2-induced oxidative stress.
Plain English Summary
Researchers evaluated the effects of an electromagnetic wave at a magnetic field intensity of 1 mT and a frequency of 50-Hz on human SH-SY5Y cells, a well-characterized immortalized neuronal cell model. ELF-EMF exposure elevated the expression of NOS and O2−, which were countered by compensatory changes in antioxidant catalase activity and enzymatic kinetic parameters related to CYP-450 and CAT activity. Cytokine gene expression was additionally evaluated and found rapidly modified by ELF-EMF exposure. When co-exposed to H2O2-induced oxidative stress, ELF-EMF proved not as well counteracted, resulting in a decline in CAT activity and a rise in O2− levels.
Why This Matters
The study used human SH-SY5Y cells, described as a well-characterized immortalized neuronal cell model, to examine ELF-EMF effects in a controlled laboratory setting. Under exposure alone, the cells appeared to mount a compensatory antioxidant response, with catalase activity and related enzymatic parameters adjusting in response to elevated NOS and O2−. When an additional oxidative stressor was introduced alongside the field exposure, this compensatory response was overcome, resulting in a decline in CAT activity and a rise in O2− levels. The researchers stated that these findings support the further evaluation of ELF-EMF exposure in cellular and in vivo preclinical models to define mechanisms potentially impacted in humans.
Exposure Details
- Magnetic Field
- 1 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 24 hours (1 h, 3 h, 6 h or 24 h)
Exposure Context
This study used 1 mT for magnetic fields:
- 50Kx above the Building Biology guideline of 0.2 mG
- 10Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
We evaluated the effects of an electromagnetic wave (magnetic field intensity 1mT; frequency, 50-Hz) on a well-characterized immortalized neuronal cell model, human SH-SY5Y cells.
ELF-EMF exposure elevated the expession of NOS and O2−, which were countered by compensatory changes...
Together these studies support the further evaluation of ELF-EMF exposure in cellular and in vivo preclinical models to define mechanisms potentially impacted in humans.
Show BibTeX
@article{m_2014_neuronal_cellular_responses_to_289,
author = {Reale M and Kamal MA and Patruno A and Costantini E and D'Angelo C and Pesce M and Greig NH},
title = {Neuronal Cellular Responses to Extremely Low Frequency Electromagnetic Field Exposure: Implications Regarding Oxidative Stress and Neurodegeneration.},
year = {2014},
url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0104973},
}