Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media.
Pakhomova ON, Khorokhorina VA, Bowman AM, Rodaitė-Riševičienė R, Saulis G, Xiao S, Pakhomov AG · 2012
View Original AbstractNanosecond pulsed electric field exposure generated both extracellular and intracellular reactive oxygen species, including hydrogen peroxide, in cells and cell-free media.
Plain English Summary
Researchers assessed reactive oxygen species (ROS) production in cultured cells and media exposed to 300-ns pulsed electric fields at 1 to 13 kV/cm. In cell-free media, the pulsed electric field facilitated conversion of H2DCF into the fluorescent marker DCF, an effect reduced by catalase but not by mannitol or superoxide dismutase, and not related to heating. The researchers concluded that bioeffects of nanosecond pulsed electric fields are not limited to electropermeabilization, and that concurrent ROS formation may lead to cell stimulation and/or oxidative cell damage.
Why This Matters
In cell suspensions, the fluorescent signal increased in proportion to the number of pulses and the density of cells present. DCF emission continued to rise over time after exposure in nsPEF-sensitive Jurkat cells, but remained stable in nsPEF-resistant U937 cells, suggesting that cell type influenced the oxidative response. Formation of hydrogen peroxide in treated media was separately confirmed by increased oxidation of Amplex Red, and ROS increase within individual cells was visualized by oxidation of DHE. The researchers framed oxidative effects as a possible separate primary mechanism responsible for bioeffects, distinct from the better-known effect of electropermeabilization.
Exposure Details
- Electric Field
- 100000 -1300000 V/m
- Source/Device
- 300-ns PEF
Exposure Context
This study used 100000 -1300000 V/m for electric fields:
- 333.3Kx above the Building Biology guideline of 0.3 V/m
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Study Details
We hypothesized that oxidative effects of nsPEF could be a separate primary mechanism responsible for bioeffects.
ROS production in cultured cells and media exposed to 300-ns PEF (1–13 kV/cm) was assessed by oxidat...
When a suspension of H2DCF-loaded cells was subjected to nsPEF, the yield of fluorescent 2′,7′-dichl...
Show BibTeX
@article{on_2012_oxidative_effects_of_nanosecond_436,
author = {Pakhomova ON and Khorokhorina VA and Bowman AM and Rodaitė-Riševičienė R and Saulis G and Xiao S and Pakhomov AG},
title = {Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media.},
year = {2012},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0003986112003037},
}