Pulsed or continuous electromagnetic field induce p53/p21-mediated apoptotic signaling pathway in mouse spermatogenic cells in vitro and thus may affect male fertility.
Solek P, Majchrowicz L, Bloniarz D, Krotoszynska E, Koziorowski M. · 2017
View Original AbstractPulsed and continuous electromagnetic fields induced DNA damage and apoptosis in mouse spermatogenic cells in vitro, suggesting possible effects on male fertility.
Plain English Summary
Researchers investigated the effects of pulsed and continuous electromagnetic fields on two mouse spermatogenic cell lines, GC-1 spg and GC-2 spd, in vitro. The researchers evaluated mitochondrial metabolism, morphology, proliferation rate, viability, cell cycle progression, oxidative stress balance, and regulatory proteins. The results strongly suggested that EMF induced oxidative and nitrosative stress-mediated DNA damage, resulting in p53/p21-dependent cell cycle arrest and apoptosis. The researchers concluded that electromagnetic field impairs male fertility by inducing p53/p21-mediated cell cycle arrest and apoptosis.
Why This Matters
The study examined two established mouse spermatogenic cell lines, GC-1 spg and GC-2 spd, in a laboratory setting in vitro. Both pulsed and continuous forms of electromagnetic field were tested on the same cell lines. The researchers proposed that spermatogenic cells may be especially vulnerable because they lack sufficient antioxidant enzymes to counteract oxidative and nitrosative stress. These cytotoxic and genotoxic events were described as contributing to infertility through a reduction in the healthy sperm cells pool.
Exposure Details
- Magnetic Field
- 2.5 and 8 mT
- Source/Device
- 2 Hz,50 Hz, 120 Hz
- Exposure Duration
- 2 hours
Exposure Context
This study used 2.5 and 8 mT for magnetic fields:
- 125Kx above the Building Biology guideline of 0.2 mG
- 25Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
In the present study, we investigated the effects of pulsed and continuous electromagnetic field (PEMF/CEMF) on mouse spermatogenic cell lines (GC-1 spg and GC-2 spd) in terms of cellular and biochemical features in vitro.
We evaluated the effect of EMF on mitochondrial metabolism, morphology, proliferation rate, viabilit...
Our results strongly suggest that EMF induces oxidative and nitrosative stress-mediated DNA damage, ...
In conclusion, electromagnetic field present in surrounding environment impairs male fertility by inducing p53/p21-mediated cell cycle arrest and apoptosis.
Show BibTeX
@article{p_2017_pulsed_or_continuous_electromagnetic_466,
author = {Solek P and Majchrowicz L and Bloniarz D and Krotoszynska E and Koziorowski M. },
title = {Pulsed or continuous electromagnetic field induce p53/p21-mediated apoptotic signaling pathway in mouse spermatogenic cells in vitro and thus may affect male fertility.},
year = {2017},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0300483X17300926},
}