Simultaneous application of cisplatin and static magnetic field enhances oxidative stress in HeLa cell line
Kamalipooya S, Abdolmaleki P, Salemi Z, Javani Jouni F, Zafari J, Soleimani H. · 2017
View Original AbstractCombining a static magnetic field with cisplatin led to a marked decrease in HeLa cell viability by about 89%, while having small effects on normal cells.
Plain English Summary
Researchers studied the effects of combining a static magnetic field (SMF) with the anticancer drug cisplatin on oxidative stress in human cervical cancer cells (HeLa) and normal skin fibroblast cells (Hu02). Cells were exposed to SMF intensities of 7, 10, and 15 mT for 24 and 48 hours, and cytotoxic effects were measured using reactive oxygen species content and membrane lipid peroxidation. The combination of both treatments for 48 hours led to a marked decrease in HeLa cell viability of about 89% compared to untreated cells. The study suggests that among the optional intensities of SMF tested, the intensity of 10 mT led to higher damage to cancer cells at lower doses of the drug.
Why This Matters
The study examined a cancer cell line (HeLa) and normal skin fibroblast cells (Hu02) side by side, allowing researchers to compare how each responded to the combined treatment. The highest death rate was observed in HeLa cells and the lowest in Hu02 cells, both at the intensity of 10 mT. The SMF appeared to sensitize cancer cells to cisplatin through the accumulation of reactive oxygen species, while having small effects on normal cells. Membrane lipid peroxidation was found to be higher in cancer cells than in normal counterparts. The researchers suggest that combining physical and chemical treatments could increase oxidative stress specifically in the HeLa cell line.
Exposure Details
- Magnetic Field
- 7, 10, and 15 mT
- Exposure Duration
- 24 and 48 h
Exposure Context
This study used 7, 10, and 15 mT for magnetic fields:
- 350Kx above the Building Biology guideline of 0.2 mG
- 70Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
In this study, we reported the effects of simultaneous application of static magnetic field (SMF) and cisplatin as an anticancer drug on the oxidative stress in human cervical cancer (HeLa) cell line and normal skin fibroblast cells (Hu02).
The cells were exposed to different SMF intensities (7, 10, and 15 mT) for 24 and 48 h. IC50 concent...
Based on the obtained results, the highest and lowest death rate, respectively, in HeLa and Hu02 cel...
This study suggests that conjugation of both physical and chemical treatments could increase the oxidative stress in HeLa cell line and among three optional intensities of SMF, the intensity of 10 mT led to the higher damage to cancer cells in lower doses of drug.
Show BibTeX
@article{s_2017_simultaneous_application_of_cisplatin_393,
author = {Kamalipooya S and Abdolmaleki P and Salemi Z and Javani Jouni F and Zafari J and Soleimani H.},
title = {Simultaneous application of cisplatin and static magnetic field enhances oxidative stress in HeLa cell line},
year = {2017},
doi = {10.1007/s11626-017-0148-z},
url = {https://link.springer.com/article/10.1007/s11626-017-0148-z},
}