Comparison of the Genotoxic Effects Induced by 50 Hz Extremely Low-Frequency Electromagnetic Fields and 1800 MHz Radiofrequency Electromagnetic Fields in GC-2 Cells.
Duan W, Liu C, Zhang L, He M, Xu S, Chen C, Pi H, Gao P, Zhang Y, Zhong M, Yu Z, Zhou Z. · 2015
View Original AbstractAt high intensities, 50 Hz ELF-EMF caused DNA strand breaks in mouse spermatocyte-derived cells, while 1800 MHz RF-EMF caused oxidative DNA base damage, suggesting different damage patterns.
Plain English Summary
Researchers exposed a mouse spermatocyte-derived GC-2 cell line intermittently to 50 Hz ELF-EMF at intensities of 1, 2, or 3 mT, or to 1800 MHz RF-EMF in GSM-Talk mode at SAR values of 1, 2, or 4 W/kg. After 24 hours of exposure, neither ELF-EMF nor RF-EMF affected cell viability. ELF-EMF exposure significantly increased DNA strand breaks at 3 mT, while RF-EMF exposure significantly induced oxidative DNA base damage at a SAR of 4 W/kg. The researchers suggest that both field types may produce genotoxicity at relatively high intensities but through different patterns of DNA damage.
Why This Matters
The study used standardized exposure conditions so that results from the two field types could be directly compared. DNA damage was detected using an alkaline comet assay and immunofluorescence against γ-H2AX foci, as well as a formamidopyrimidine DNA glycosylase (FPG)-modified alkaline comet assay for oxidative base damage. The ELF-EMF and RF-EMF exposures produced different types of DNA damage, leading the researchers to suggest that the underlying mechanisms of genotoxicity may differ between the two field types.
Exposure Details
- Magnetic Field
- 1,2,3 mT
- Source/Device
- 50 Hz ELF-EMF
- Exposure Duration
- intermittently (5 min on and 10 min off)
Exposure Context
This study used 1,2,3 mT for magnetic fields:
- 50Kx above the Building Biology guideline of 0.2 mG
- 10Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
The aim of this study is to observe the Comparison of the Genotoxic Effects Induced by 50 Hz Extremely Low-Frequency Electromagnetic Fields and 1800 MHz Radiofrequency Electromagnetic Fields in GC-2 Cells
To make experiments controllable and results comparable, we standardized exposure conditions and exp...
After exposure for 24 h, we found that neither ELF-EMF nor RF-EMF affected cell viability using Cell...
Our results suggest that both ELF-EMF and RF-EMF under the same experimental conditions may produce genotoxicity at relative high intensities, but they create different patterns of DNA damage. Therefore, the potential mechanisms underlying the genotoxicity of different frequency electromagnetic fields may be different.
Show BibTeX
@article{w_2015_comparison_of_the_genotoxic_522,
author = {Duan W and Liu C and Zhang L and He M and Xu S and Chen C and Pi H and Gao P and Zhang Y and Zhong M and Yu Z and Zhou Z.},
title = {Comparison of the Genotoxic Effects Induced by 50 Hz Extremely Low-Frequency Electromagnetic Fields and 1800 MHz Radiofrequency Electromagnetic Fields in GC-2 Cells.},
year = {2015},
url = {https://meridian.allenpress.com/radiation-research/article-abstract/183/3/305/150481/Comparison-of-the-Genotoxic-Effects-Induced-by-50},
}