An evaluation of genotoxicity in human neuronal-type cells subjected to oxidative stress under an extremely low frequency pulsed magnetic field.
Giorgi G, Lecciso M, Capri M, Lukas Yani S, Virelli A, Bersani F, Del Re B. · 2014
View Original AbstractPulsed magnetic field exposure did not interfere with DNA damage or cell toxicity caused by oxidative stress in human neuronal-type cells.
Plain English Summary
Researchers investigated whether pulsed magnetic field exposure could interfere with DNA damage and repair in human neuroblastoma BE(2)C cells under oxidative stress. Cells were treated with hydrogen peroxide and exposed to a pulsed magnetic field at 1 mT and 50 Hz, and DNA double strand breaks were tracked using gamma-H2AX foci formation at 1, 24, 48, and 72 hours after treatment. The results suggest that pulsed magnetic field exposure does not interfere with genotoxicity and cytotoxicity induced by oxidative stress.
Why This Matters
The study used gamma-H2AX foci formation as a marker of DNA double strand breaks, described as a sensitive indicator of this type of damage. Cytotoxicity was also assessed using an MTT assay and cell cycle analysis at various time points after treatment.
Exposure Details
- Magnetic Field
- 1 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 1, 24, 48, 72 h
Exposure Context
This study used 1 mT for magnetic fields:
- 50Kx above the Building Biology guideline of 0.2 mG
- 10Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
The aim of this study is to investigate whether PMF exposure can interfere with DNA damage and repair in the presence of a genotoxic oxidative agent in neuronal type cells.
To this purpose gamma-H2AX foci formation, which is a sensitive marker of DNA double strand breaks (...
Taken together, results suggest that PMF exposure does not interfere with genotoxicity and cytotoxic...
Show BibTeX
@article{g_2014_an_evaluation_of_genotoxicity_253,
author = {Giorgi G and Lecciso M and Capri M and Lukas Yani S and Virelli A and Bersani F and Del Re B.},
title = {An evaluation of genotoxicity in human neuronal-type cells subjected to oxidative stress under an extremely low frequency pulsed magnetic field.},
year = {2014},
url = {https://www.sciencedirect.com/science/article/abs/pii/S1383571814002721},
}