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Reactive oxygen species (ROS) production in human peripheral blood neutrophils exposed in vitro to static magnetic field.

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Poniedziałek B, Rzymski P, Karczewski J, Jaroszyk F, Wiktorowicz K · 2013

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Exposing human neutrophils to a static magnetic field changed reactive oxygen species production in ways that depended on exposure time and field orientation.

Plain English Summary

Summary written for general audiences

Researchers exposed human peripheral blood samples to a gradient static magnetic field (SMF) in vitro to measure its effect on reactive oxygen species (ROS) production in neutrophils. Blood samples from healthy individuals were incubated in an inhomogeneous SMF at either the south or north pole for 15, 30, or 45 minutes, with a maximum field induction of approximately 60 mT. The exposure induced statistically significant changes in ROS production in both unstimulated and PMA-stimulated neutrophils, with effects that were highly correlated with exposure time and depended on field orientation.

Why This Matters

ROS production was measured using dihydrorhodamine (123DHR) as a fluorophore, with phorbol 12-myristate 13-acetate (PMA) used as a respiratory burst stimulator, and 123DHR oxidation was detected by flow cytometry. The researchers noted that the intracellular mechanisms behind these interactions are not thoroughly understood, but suggested that SMF may affect ROS metabolic oscillations and their formation and inactivation. The study highlighted the importance of properly adjusting exposure time to SMF for any potential therapeutic applications.

Exposure Details

Magnetic Field
60 mT
Exposure Duration
15, 30 or 45 minutes

Exposure Context

This study used 60 mT for magnetic fields:

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Exposure Level in ContextStudy Exposure Level in ContextThis study: 60 mTExtreme Concern - 5 mGFCC Limit - 2,000 mGEffects observed in the Extreme Concern range (Building Biology)This level is 300 times the FCC limit

Study Details

The aim of this study was to determine the effect of gradient static magnetic field (SMF) on reactive oxygen species (ROS) production in human neutrophils in peripheral blood in vitro.

Blood samples collected from healthy individuals were incubated in an inhomogeneous SMF (in a south ...

The exposure of blood samples to SMF induced statistically significant changes in ROS production in ...

This study emphasizes the importance of proper adjustment of exposure time to SMF for any potential therapeutic applications.

Cite This Study
Poniedziałek B, Rzymski P, Karczewski J, Jaroszyk F, Wiktorowicz K (2013). Reactive oxygen species (ROS) production in human peripheral blood neutrophils exposed in vitro to static magnetic field. Electromagn Biol Med. 32(4):560-568, 2013b.
Show BibTeX
@article{b_2013_reactive_oxygen_species_ros_444,
  author = {Poniedziałek B and Rzymski P and Karczewski J and Jaroszyk F and Wiktorowicz K},
  title = {Reactive oxygen species (ROS) production in human peripheral blood neutrophils exposed in vitro to static magnetic field.},
  year = {2013},
  doi = {10.3109/15368378.2013.773910},
  url = {https://www.tandfonline.com/doi/abs/10.3109/15368378.2013.773910},
}
DOI: 10.3109/15368378.2013.773910

Cited By (19 papers)

Quick Questions About This Study

The study used an inhomogeneous gradient static magnetic field with a maximum induction of approximately 60 mT, applied at either the south or north pole.
The exposure induced statistically significant changes in ROS production in both unstimulated and PMA-stimulated neutrophils.
The observed effects were highly correlated with exposure time and also depended on the orientation of the field.
Researchers used dihydrorhodamine (123DHR) as a fluorophore, and measured its oxidation by ROS via flow cytometry.
The researchers noted that intracellular mechanisms underlying such interactions are not thoroughly understood, but suggested that SMF may affect ROS metabolic oscillations and their formation and inactivation.