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Cellular Effects

Mitochondrial ROS release and subsequent Akt Activation potentially mediated the anti-apoptotic effect of a 50-Hz magnetic field on FL cells.

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Feng B, Ye C, Qiu L, Chen L, Fu Y, Sun W · 2016

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A 50-Hz magnetic field protected human amniotic FL cells against staurosporine-induced apoptosis, an effect that depended on mitochondrial ROS release and Akt activation.

Plain English Summary

Summary written for general audiences

Human amniotic FL cells were exposed to a 50-Hz magnetic field for different durations or intensities, then incubated with staurosporine for 4 hours. Magnetic field exposure alone had no significant effect on early apoptosis or cell viability. When cells were also treated with staurosporine, magnetic field exposure had a protective effect against early apoptosis, and this effect could be abolished by MPT inhibitors. The anti-apoptotic effect was completely eliminated when Akt was inhibited, suggesting that Akt activation was necessary for the effect.

Why This Matters

Researchers measured early apoptosis, cell viability, mitochondrial ROS, and levels of phosphorylated Akt after magnetic field exposure. Exposing cells to the magnetic field increased mitochondrial ROS, which were released into the cytoplasm through mitochondrial permeability transition pores. This ROS release was associated with ROS-dependent phosphorylation of Akt. The study indicated a possibility that mitochondrial ROS release through mPTP and subsequent Akt activation were necessary for the anti-apoptotic effect.

Exposure Details

Magnetic Field
0.4 mT
Source/Device
50-Hz
Exposure Duration
5, 15, 30, 60 or 120 min

Exposure Context

This study used 0.4 mT for magnetic fields:

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Exposure Level in ContextStudy Exposure Level in ContextThis study: 0.4 mTExtreme Concern - 5 mGFCC Limit - 2,000 mGEffects observed in the Extreme Concern range (Building Biology)This level is 2 times the FCC limit
A logarithmic frequency spectrum from 10 Hz to 100 GHz showing where this study's 50 Hz exposure sits relative to common EMF sources.Where This Frequency Sits on the EMF SpectrumELFVLFLF / MFHF / VHFUHFSHFmm10 Hz100 GHzThis study: 50 HzCell phones~1 GHzWiFi2.4 GHz5G mm28 GHzLogarithmic scale

Study Details

In the present study, the aim was to explore the possible biological implications of MF-induced transient MPT

Human amniotic (FL) cells were exposed to MF for different durations or intensities followed by incu...

MF exposure had a protective effect against early apoptosis induced by staurosporine, which could be...

the present study indicated a possibility that mitochondrial ROS release through mPTP and subsequent Akt activation were necessary for the anti-apoptotic effect of MF.

Cite This Study
Feng B, Ye C, Qiu L, Chen L, Fu Y, Sun W (2016). Mitochondrial ROS release and subsequent Akt Activation potentially mediated the anti-apoptotic effect of a 50-Hz magnetic field on FL cells. Cell Physiol Biochem. 38(6):2489-2499, 2016c.
Show BibTeX
@article{b_2016_mitochondrial_ros_release_and_362,
  author = {Feng B and Ye C and Qiu L and Chen L and Fu Y and Sun W},
  title = {Mitochondrial ROS release and subsequent Akt Activation potentially mediated the anti-apoptotic effect of a 50-Hz magnetic field on FL cells.},
  year = {2016},
  
  url = {https://www.karger.com/Article/Abstract/445599},
}

Quick Questions About This Study

Human amniotic FL cells were exposed to a magnetic field for different durations or intensities.
Magnetic field exposure alone had no significant effect on early apoptosis or cell viability.
Magnetic field exposure increased mitochondrial ROS released into the cytoplasm through mPTP, and this was associated with ROS-dependent phosphorylation of Akt.
The anti-apoptotic effect of magnetic field exposure was completely eliminated when Akt was inhibited.
The study indicated a possibility that mitochondrial ROS release through mPTP and subsequent Akt activation were necessary for the anti-apoptotic effect of the magnetic field.