Power frequency magnetic fields induced reactive oxygen species-related autophagy in mouse embryonic fibroblasts.
Chen Y, Hong L, Zeng Y, Shen Y, Zeng Q. · 2014
View Original AbstractExposing mouse embryonic fibroblasts to a 50 Hz magnetic field at 2 mT increased autophagic markers at 6 hours, with reactive oxygen species involved in the process.
Plain English Summary
Researchers exposed mouse embryonic fibroblasts to a 50 Hz power frequency magnetic field at 2 mT across several time points and measured autophagy markers. A significant increase in autophagic markers was observed at 6 hours, including higher LC3-II levels, increased GFP-LC3 puncta formation, and increased numbers of autophagic vacuoles. The increase in autophagic markers was the result of enhanced autophagic flux and not the suppression of lysosomal function, and the autophagic process involved reactive oxygen species and was independent of the mTOR signaling pathway.
Why This Matters
The study measured autophagic markers including LC3-II protein levels, GFP-LC3 puncta formation, and autophagic vacuoles visible under a transmission electron microscope. Using chloroquine, the researchers provided evidence that the increase of autophagic markers was the result of enhanced autophagic flux and not the suppression of lysosomal function. The autophagic process involved reactive oxygen species and was independent of the mammalian target of rapamycin signaling pathway.
Exposure Details
- Magnetic Field
- 2 mT
- Source/Device
- 50 Hz PFMF
- Exposure Duration
- 0.5 h, 2 h, 6 h, 12 h, and 24 h
Exposure Context
This study used 2 mT for magnetic fields:
- 100Kx above the Building Biology guideline of 0.2 mG
- 20Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
In this study, we investigated the effects of PFMF on mouse embryonic fibroblasts (MEF) autophagy.
After cells were exposed to 50 Hz PFMF at 2 mT for 0.5 h, 2 h, 6 h, 12 h, and 24 h, we observed a si...
Show BibTeX
@article{y_2014_power_frequency_magnetic_fields_329,
author = {Chen Y and Hong L and Zeng Y and Shen Y and Zeng Q.},
title = {Power frequency magnetic fields induced reactive oxygen species-related autophagy in mouse embryonic fibroblasts.},
year = {2014},
url = {https://www.sciencedirect.com/science/article/abs/pii/S1357272514003240},
}