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Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field.

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Cheun BS, Yi SH, Baik KY, Lim JK, Yoo JS, Shin HW, Soh KS · 2007

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Exposing canine kidney cells to a 60 Hz AC magnetic field changed the decay pattern of chemiluminescence intensity compared to unexposed cells.

Plain English Summary

Summary written for general audiences

Researchers studied biophoton emission from Madin-Darby canine kidney (MDCK) cells treated with hydrogen peroxide, with and without exposure to a 60 Hz AC magnetic field. Hydrogen peroxide increased biophoton emission from the cells, and the increase was dependent on the amount of hydrogen peroxide applied. When the 60 Hz AC magnetic field was applied, the decay of chemiluminescence intensity took a different path compared to cells not exposed to the field.

Why This Matters

The study used a photomultiplier tube (PMT) and a fluorescence microscope to detect and measure ultraweak light emitted by the cells. Hydrogen peroxide served as a way to produce reactive oxygen species (ROS) in the cell cultures. Results from the PMT and the fluorescence microscope agreed with each other, which the researchers said suggests the possibility of quantitative measurement of ROS influence on living tissue or cells.

Exposure Details

Magnetic Field
1.4 mT
Source/Device
60 Hz AC Magnetic Field
Exposure Duration
2 h up to 3 h 40 min

Exposure Context

This study used 1.4 mT for magnetic fields:

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Exposure Level in ContextStudy Exposure Level in ContextThis study: 1.4 mTExtreme Concern - 5 mGFCC Limit - 2,000 mGEffects observed in the Extreme Concern range (Building Biology)This level is 7 times the FCC limit
A logarithmic frequency spectrum from 10 Hz to 100 GHz showing where this study's 60 Hz exposure sits relative to common EMF sources.Where This Frequency Sits on the EMF SpectrumELFVLFLF / MFHF / VHFUHFSHFmm10 Hz100 GHzThis study: 60 HzCell phones~1 GHzWiFi2.4 GHz5G mm28 GHzLogarithmic scale

Study Details

This study was performed to study biophoton charcteristics (ultraweak photon emission) of canine kidney cells under the influence of hydrogen peroxide with and without exposure to extremely low frequency magnetic field.

We studied biophoton characteristics of Madin-Darby canine kidney (MDCK) cells under the influence o...

Images from a fluorescence microscope show an increase of photon intensity emitted from the sample d...

The agreement between the results of the PMT and the fluorescence microscope suggests the possibility of quantitative measurement of the influence of ROS on living tissue or cell. Therefore a PMT can be used as an useful tool to study biochemical characteristic relating ROS. This methodology can be applied to assess environmental effects relating plant and red tide issue.

Cite This Study
Cheun BS, Yi SH, Baik KY, Lim JK, Yoo JS, Shin HW, Soh KS (2007). Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field. J Environ Biol. 28(4):735-740, 2007.
Show BibTeX
@article{bs_2007_biophoton_emission_of_mdck_330,
  author = {Cheun BS and Yi SH and Baik KY and Lim JK and Yoo JS and Shin HW and Soh KS},
  title = {Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field.},
  year = {2007},
  
  url = {http://jeb.co.in/journal_issues/200710_oct07/paper_07.pdf},
}

Quick Questions About This Study

The study used Madin-Darby canine kidney (MDCK) cells.
Images from a fluorescence microscope showed an increase of photon intensity from the cells due to hydrogen peroxide, and the increase depended on the amount applied.
The decay of chemiluminescence intensity took a different path following exposure to the magnetic field.
The researchers used a photomultiplier tube (PMT) and a fluorescence microscope to study biophoton characteristics.
The researchers noted that PMT measurement might be considered a useful tool for studying biochemical characteristics in relation to reactive oxygen species.