Magnetic field-induced oxidative stress and DNA damage in Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae
Pandir D, Sahingoz R · 2014
View Original AbstractExposure to a 1.4-Tesla magnetic field increased DNA damage and oxidative stress markers in Mediterranean flour moth larvae.
Plain English Summary
Researchers exposed Mediterranean flour moth (Ephestia kuehniella) larvae to a 1.4-Tesla magnetic field at 50 Hz for time periods ranging from 3 to 72 hours. DNA damage, measured by the comet assay, was significantly larger than in the control group at increasing exposure times. Antioxidant enzyme activities (SOD, CAT, GPx, and GST) decreased and the lipid peroxidation marker MDA increased in exposed larvae compared to controls.
Why This Matters
The study used the comet assay, a single-cell gel electrophoresis technique, to detect DNA damage quickly in individual larvae cells. Comet assay results were measured using tail DNA%, tail length, and tail moment as indicators of DNA damage. Malondialdehyde (MDA) is described as the end product of lipid peroxidation and was used as a marker of oxidative damage in larvae tissue. The researchers suggested that magnetic fields may be used as a control agent to determine potential toxic effects against E. kuehniella larvae.
Exposure Details
- Magnetic Field
- 1400 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 3, 6, 12, 24, 48, and 72 h
Exposure Context
This study used 1400 mT for magnetic fields:
- 70Mx above the Building Biology guideline of 0.2 mG
- 14Mx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
The effect of strong magnetic fields (MFs) on DNA damage and oxidative stress on larvae stage of E. kuehniella was assessed.
Antioxidant enzyme systems, which include superoxide dismutase (SOD), catalase (CAT), glutathione pe...
MFs caused increasing DNA damage and demonstrated using the comet assay with its parameters includin...
In our investigation, we showed that MFs caused oxidative stress and proved to be DNA damage as revealed by the comet assay. MFs may be used to determine potential toxic effects as a control agent against E. kuehniella larvae.
Show BibTeX
@article{d_2014_magnetic_fieldinduced_oxidative_stress_437,
author = {Pandir D and Sahingoz R},
title = {Magnetic field-induced oxidative stress and DNA damage in Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae},
year = {2014},
doi = {10.1007/s10340-013-0521-y},
url = {https://link.springer.com/article/10.1007/s10340-013-0521-y},
}