Expressions of some antioxidant genes in SH-SY5Y cells treated with β-lapachone, morphine and electromagnetic field.

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Mahmoudinasab H, Saadat M. · 2018

In SH-SY5Y cells, EMF was negatively associated with NQO1 gene expression, while morphine increased both NQO1 and NQO2 expression.

Plain English Summary

Summary written for general audiences

Researchers measured the effects of morphine and EMF, in combination with β-lapachone, on cell growth inhibition and expression of antioxidant genes in SH-SY5Y cells. The study used 0.50 mT intensity of 50 Hz EMF under two exposure conditions: "15 min field-on/15 min field-off" and "30 min field-on continuously." Under the "15 min field-on/15 min field-off" condition, NQO1 mRNA level decreased while NQO2 expression increased. Multiple linear regression analysis indicated that NQO1 mRNA levels were associated positively with β-lapachone and negatively with EMF.

Why This Matters

The study examined several antioxidant genes, including NQO1, NQO2, SOD1, SOD2, CAT, GSTO1, GSTM2, GSTM3, GSTP1, MGST1, and MGST3, in SH-SY5Y cells. Both NQO1 and NQO2 expressions increased in morphine-treated cells. The NQO1 expression level in cells treated with β-lapachone was higher than in other treatments, indicating that β-lapachone induces NQO1 expression. The IC50 values of β-lapachone in combination with morphine, EMF, and "morphine plus EMF" were higher than in cells treated only with β-lapachone. The researchers found a negative association between NQO1 mRNA levels and IC50 values of β-lapachone.

Exposure Details

Magnetic Field: 0.5 mT
Source/Device: 50 Hz
Exposure Duration: 15 min field-on/15 min field-off” and “30 min field-on continuously

Exposure Context

This study used 0.5 mT for magnetic fields:

25Kx above the Building Biology guideline of 0.2 mG
5Kx above the BioInitiative Report recommendation of 1 mG

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Details

The goal of the present study was to examine the effects of Mor and EMF, in combination with β-Lap on the cell growth inhibition and expression of several antioxidant genes.

The 0.50 mT intensity of 50 Hz EMF and two exposure conditions (“15 min field-on/15 min field-off” a...

Whereas NQO1 mRNA level decreased in the “15 min field-on/15 min field-off” condition, the expressio...

Cite This Study

Mahmoudinasab H, Saadat M. (2018). Expressions of some antioxidant genes in SH-SY5Y cells treated with β-lapachone, morphine and electromagnetic field. Mol Biol Rep. 45(3):379-387, 2018b.

Show BibTeX

@article{h_2018_expressions_of_some_antioxidant_416,
  author = {Mahmoudinasab H and Saadat M.},
  title = {Expressions of some antioxidant genes in SH-SY5Y cells treated with β-lapachone, morphine and electromagnetic field.},
  year = {2018},
  doi = {10.1007/s11033-018-4172-1},
  url = {https://link.springer.com/article/10.1007/s11033-018-4172-1},
}

DOI: 10.1007/s11033-018-4172-1

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Quick Questions About This Study

The study used SH-SY5Y cells exposed to EMF at 0.50 mT intensity of 50 Hz.

Researchers used 0.50 mT intensity of 50 Hz EMF under two conditions: "15 min field-on/15 min field-off" and "30 min field-on continuously."

NQO1 mRNA levels were associated negatively with EMF, and NQO1 mRNA level decreased under the "15 min field-on/15 min field-off" condition.

Both NQO1 and NQO2 expressions increased in morphine-treated cells.

There was a negative association between NQO1 mRNA levels and IC50 values of β-lapachone.