Induction of genomic instability, oxidative processes, and mitochondrial activity by 50Hz magnetic fields in human SH-SY5Y neuroblastoma cells.
Luukkonen J, Liimatainen A, Juutilainen J, Naarala J · 2014
View Original AbstractExposure of human neuroblastoma cells to a 50-Hz magnetic field was associated with increased micronuclei and signs of genomic instability in cell progeny days after exposure.
Plain English Summary
Researchers exposed human SH-SY5Y neuroblastoma cells to a 50-Hz, 100-μT magnetic field for 24 hours, followed by a 3-hour exposure to menadione. The study aimed to investigate cellular changes occurring during the first 24 hours of magnetic field exposure and whether those changes could lead to genomic instability in the progeny of exposed cells. Magnetic field exposure was associated with increased micronuclei at 8 and 15 days after exposure, increased mitochondrial activity at 8 days, and increased reactive oxygen species production and lipid peroxidation at 15 days. The researchers suggest that magnetic field exposure disturbs oxidative balance immediately after exposure, and that persistently elevated levels of micronuclei were found in the progeny of exposed cells.
Why This Matters
The cells used were human SH-SY5Y neuroblastoma cells, a type of nerve cell line studied in a laboratory setting. Micronuclei served as the indicator of genomic instability in this study, and elevated levels were observed not just immediately after exposure but in the progeny of exposed cells days later. Oxidative processes, including reactive oxygen species production, reduced glutathione level, and mitochondrial superoxide level, were affected immediately after the magnetic field exposure. The researchers suggest the immediate disturbance of oxidative balance might explain previously observed effects on cellular responses to menadione-induced DNA damage.
Exposure Details
- Magnetic Field
- 0.1 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 24 h
Exposure Context
This study used 0.1 mT for magnetic fields:
- 5Kx above the Building Biology guideline of 0.2 mG
- 1Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
The aim of this study was to investigate the cellular changes that must occur already during the first 24 h of exposure to MF, and to explore whether the MF-induced changes in DNA damage response can lead to genomic instability in the progeny of the exposed cells.
In order to answer these questions, human SH-SY5Y neuroblastoma cells were exposed to a 50-Hz, 100-μ...
The main finding was that MF exposure was associated with increased level of micronuclei, used as an...
In conclusion, the present results suggest that MF exposure disturbs oxidative balance immediately after the exposure, which might explain our previous findings on MF altered cellular responses to menadione-induced DNA damage. Persistently elevated levels of micronuclei were found in the progeny of MF-exposed cells, indicating induction of genomic instability.
Show BibTeX
@article{j_2014_induction_of_genomic_instability_413,
author = {Luukkonen J and Liimatainen A and Juutilainen J and Naarala J},
title = {Induction of genomic instability, oxidative processes, and mitochondrial activity by 50Hz magnetic fields in human SH-SY5Y neuroblastoma cells. },
year = {2014},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0027510713001991},
}