Effect of electric fields on growth rate of embryonic chick tibiae in vitro
L. E. DAVIS, SUSAN SHURIN, R. T. JOHNSON · 1975
Pulsed electric fields affected growth of embryonic chick tibiae in vitro, but static fields did not.
Plain English Summary
Researchers cultured embryonic chick tibiae in vitro and exposed them to either a static or a pulsed electric field. Histological examination and macroscopic photographs showed no differences in growth patterns between test and control bones.
Why This Matters
Tibiae from 8- to 9-day-old embryos were aseptically excised and placed on a siliconised cellulose acetate mat over a sterile sponge. The 9 d incubation period at 37 °C was determined by prior trials measuring bone length until growth became asymptotic. The pulsed field used a square wave with approximately a 1:10 mark-space ratio generated by switching the upper plate between -1,400 V and ground.
Finding
We report that the gross development of embryonic chick tibiae grown in vitro is affected by a pulsed, transverse electric field, but that no significant changes were observed when a static, non-varying field was used.
In their words
“We report that the gross development of embryonic chick tibiae grown in vitro is affected by a pulsed, transverse electric field, but that no significant changes were observed when a static, non-varying field was used.”
Show BibTeX
@article{effect_of_electric_fields_on_growth_rate_of_embryonic_chick_tibiae_in_vitro_g5713,
author = {L. E. DAVIS and SUSAN SHURIN and R. T. JOHNSON},
title = {Effect of electric fields on growth rate of embryonic chick tibiae in vitro},
year = {1975},
}