Effects of extremely low-frequency electromagnetic field on expression levels of some antioxidant genes in human MCF-7 cells.
Mahmoudinasab H, Sanie-Jahromi F, Saadat M · 2016
View Original AbstractAt the 15-min-on/15-min-off exposure pattern, the 0.50 mT intensity was associated with more up- or down-regulated antioxidant genes than 0.25 mT in human MCF-7 cells.
Plain English Summary
Researchers designed the study to evaluate the effect of EMFs on the mRNA levels of seven antioxidant genes in human MCF-7 cells. Three exposure patterns were tested at two field intensities, 0.25 mT and 0.50 mT, each with a total exposure time of 30 minutes. The MTT assay showed no cytotoxic effect on MCF-7 cells under any of the three exposure conditions at either field intensity. Under the 15-min-on/15-min-off pattern, the 0.50 mT intensity was associated with more up- or down-regulated genes than 0.25 mT, a statistically significant difference (P=0.041), while no such difference was found for the other two exposure patterns.
Why This Matters
The seven antioxidant genes studied were CAT, SOD1, SOD2, GSTO1, GSTM3, MSGT1, and MSGT3. Gene expression was measured using quantitative real-time polymerase chain reaction, with RNA extracted immediately after exposure and again two hours later. Control cultures were placed in the same exposure apparatus with the power switched off. The statistically significant difference in gene regulation between the two field intensities applied only to the 15-min-on/15-min-off pattern and not to the other two exposure conditions.
Exposure Details
- Magnetic Field
- 0.25 and 0.50 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 30 minutes
Exposure Context
This study used 0.25 and 0.50 mT for magnetic fields:
- 12.5Kx above the Building Biology guideline of 0.2 mG
- 2.5Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
This study was designed to evaluate the effect of 50-Hz EMFs on the mRNA levels of seven antioxidant genes (CAT, SOD1, SOD2, GSTO1, GSTM3, MSGT1, and MSGT3) in human MCF-7 cells.
The EMF exposure patterns were: 1) 5 min field-on/5 min filed-off, 2) 15 min field-on/15 min field-o...
MTT assay for three exposure conditions in the two field intensities represented no cytotoxic effect...
Show BibTeX
@article{h_2016_effects_of_extremely_lowfrequency_414,
author = {Mahmoudinasab H and Sanie-Jahromi F and Saadat M},
title = {Effects of extremely low-frequency electromagnetic field on expression levels of some antioxidant genes in human MCF-7 cells.},
year = {2016},
url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5219897/},
}