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Pulsed electric field exposure of insulin induces anti-proliferative effects on human hepatocytes

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Authors not listed · 2005

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Pulsed electric field exposure of insulin reduced its receptor binding capacity to 87% of control and decreased intracellular tyrosine phosphorylation by 11% in human hepatocytes.

Plain English Summary

Summary written for general audiences

Researchers exposed insulin solution to a pulsed electric field of 0.7 V/m at 50 Hz for 20 minutes and then added it to human hepatocyte cultures. They investigated insulin signaling events including ligand-receptor binding, tyrosine phosphorylation, gene transcription, and cell proliferation using multiple methods. PEF exposure caused a conformational change in insulin, reduced its receptor binding capacity to 87% of control, and decreased intracellular tyrosine phosphorylation by 11%. The expression of 55 out of 12000 genes was altered, including increased expression of human tyrosine phosphatase and a small GTP-binding protein.

Why This Matters

The study aimed to examine how pulsed electric fields affect insulin and its role in proliferative changes in human hepatocytes. The researchers proposed a mechanism linking PEF-induced insulin changes to hepatic cell proliferation via the insulin signaling pathway.

Exposure Information

A logarithmic frequency spectrum from 10 Hz to 100 GHz showing where this study's 50 Hz exposure sits relative to common EMF sources.Where This Frequency Sits on the EMF SpectrumELFVLFLF / MFHF / VHFUHFSHFmm10 Hz100 GHzThis study: 50 HzCell phones~1 GHzWiFi2.4 GHz5G mm28 GHzLogarithmic scale

Specific exposure levels were not quantified in this study.

Cite This Study
Unknown (2005). Pulsed electric field exposure of insulin induces anti-proliferative effects on human hepatocytes.
Show BibTeX
@article{pulsed_electric_field_exposure_of_insulin_induces_anti_proliferative_effects_on_human_hepatocytes_ce1462,
  author = {Unknown},
  title = {Pulsed electric field exposure of insulin induces anti-proliferative effects on human hepatocytes},
  year = {2005},
  doi = {10.1002/bem.20156},
  
}
DOI: 10.1002/bem.20156PubMed: 16189829

Quick Questions About This Study

The pulsed electric field had an intensity of about 0.7 V/m at 50 Hz, and insulin solution was exposed for 20 minutes before being added to human hepatocyte cultures.
PEF exposure reduced the binding capacity of insulin to its receptors to 87% of the control level.
The expression of 55 out of 12000 genes was modified, including increased expression of human tyrosine phosphatase and a small GTP-binding protein.
They proposed a mechanism to explain the bio-effects of PEF on hepatic cell proliferation through the insulin signaling pathway.
The researchers used fluorescence spectroscopy, immunocytochemistry, microarrays, RT-PCR, and MTT to study ligand-receptor binding, tyrosine phosphorylation, gene transcription, and cell proliferation.