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Studies on influencing the activity of cellular enzymes by irradiation with high-frequency electromagnetic waves

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G. Ohlenschläger, I. Beyer, W. Gruno · 1972

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1972 research exposed cellular enzymes to electromagnetic waves from 40 kHz to 2400 MHz and found frequency-dependent decreases and increases in enzyme activity, including irreversible decreases.

Plain English Summary

Summary written for general audiences

German researchers in 1972 exposed cellular enzymes to electromagnetic waves ranging from 40 kHz to 2400 MHz at generator output voltages between 300 and 1000 mV and observed irreversible, exposure-time-independent decreases in enzyme activity as well as frequency-dependent decreases and increases. The study discussed direct hits in the active center of the enzyme molecule and allosteric effects as possible mechanisms.

Why This Matters

This 1972 study from the Chemical-Physiological Institute of the University of Frankfurt tested how electromagnetic waves influence cellular enzymes. The frequency range ran from 40 kHz to 2400 MHz, and the researchers saw both inhibitions and activations of enzyme activity that depended on frequency. For aldolase, the stronger inhibitions, which further tests showed to be irreversible, were found around 100, 300 and 1050 MHz, and activations around 175 and 850 MHz. The authors state that the energies in their experiments were below 1.5 eV, far below the destruction limit of most biological elementary units, and they discuss direct hits at the active center and allosteric effects as candidate mechanisms. The enzymes were irradiated in electrode cells with platinum electrodes in direct contact with the biological material, so the results describe enzyme activity under laboratory conditions.

Figures from the Original Paper

Diagrams extracted from the original research document.

diagramPage 2 - AI-described figure: A diagram showing a microwave generator model SM2000 with its controls and specifications.
graphPage 4 - AI-described figure: Frequency-dependent inhibitions and activations of LAP from swine muscle in a buffer solution (50 mM Phosphate buffer pH 7.2) at exposure times of 10 min and 20 min.
graphPage 5 - AI-described figure: Graphs showing frequency-dependent inhibition of GOT (glutamic-oxaloacetic transaminase) activity in a buffer solution with added L-aspartate, referencing Figures 4 and 5.
Cite This Study
G. Ohlenschläger, I. Beyer, W. Gruno (1972). Studies on influencing the activity of cellular enzymes by irradiation with high-frequency electromagnetic waves.
Show BibTeX
@article{studies_on_influencing_the_activity_of_cellular_enzymes_by_irradiation_with_high_g6425,
  author = {G. Ohlenschläger and I. Beyer and W. Gruno},
  title = {Studies on influencing the activity of cellular enzymes by irradiation with high-frequency electromagnetic waves},
  year = {1972},
  
  
}
No DOI on file for this study.

Quick Questions About This Study

The study tested six enzymes: lactate dehydrogenase (LDH), leucine aminopeptidase (LAP), aldolase (ALD), glutamate-oxaloacetate transaminase (GOT), glutamate-pyruvate transaminase (GPT), and alkaline phosphatase (AP). Irradiation changed enzyme activity in frequency-dependent ways, with both decreases and increases.
The researchers exposed enzymes to electromagnetic waves from 40 kHz to 2400 MHz. Within this range they observed frequency-dependent inhibitions as well as activations of enzyme activity.
The study used generator output voltages between 300 and 1000 mV with 60-ohm matching and an electrode distance of 10 mm.
In certain time ranges the study observed exposure-time-independent irreversible inhibition of enzyme activity. For aldolase, the stronger inhibitions around 100, 300 and 1050 MHz were shown by further tests to be irreversible, while activations also occurred at some frequencies.
The study discusses two possible mechanisms: direct physical hits in the active center of the enzyme molecule and allosteric effects.