Activating Transcription Factor 4 regulation of radiofrequency radiation-induced ferroptosis in osteoblasts
Wang H, Zou W, Ding C, Cao Y · 2025
RF radiation at 150 μW/cm² elicited the most pronounced effects including reduced osteoblast viability and disturbances in iron homeostasis, and ATF4 knockdown exacerbated these effects while overexpression conferred protection.
Plain English Summary
Researchers exposed MC3T3-E1 osteoblasts to radiofrequency radiation at intensities of 50, 150, and 450 μW/cm². The 150 μW/cm² exposure caused the greatest reduction in cell viability, increased lipid peroxidation, and disturbed iron homeostasis. ATF4 knockdown worsened these effects, while ATF4 overexpression protected the cells from radiation-induced damage.
Why This Matters
Bioinformatic analysis of public gene databases linked differentially expressed genes to lipid metabolism and ferroptosis pathways. The researchers performed ATF4 knockdown and overexpression experiments to test its role in RF-induced ferroptosis. The study demonstrates the crucial role of ATF4 modulation in RF radiation-induced ferroptosis in osteoblasts, a process potentially contributing to bone disorders such as osteoporosis and impaired fracture healing.
Show BibTeX
@article{wang_h_zou_w_ding_c_cao_y_ce2635,
author = {Wang H and Zou W and Ding C and Cao Y},
title = {Activating Transcription Factor 4 regulation of radiofrequency radiation-induced ferroptosis in osteoblasts},
year = {2025},
doi = {10.1080/15368378.2025.2547799},
}