Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach.
Morabito C, Rovetta F, Bizzarri M, Mazzoleni G, Fanò G, Mariggiò MA · 2010
View Original AbstractExtremely low frequency electromagnetic fields altered oxidative stress markers and calcium handling in C2C12 muscle cells.
Plain English Summary
Researchers used C2C12 cells as an in vitro model of the skeletal muscle phenotype to study the effects of short-term exposure to extremely low frequency electromagnetic fields on muscle cell differentiation and function. ELF-EMFs induced reactive oxygen species production in myoblasts and myotubes with a concomitant decrease in mitochondrial membrane potential. The cellular detoxification system was activated, with increases in catalase and glutathione peroxidase activities. ELF-EMFs also altered intracellular calcium homeostasis, increasing the spontaneous activity of myotubes and enhancing cellular reactivity to a depolarizing agent or an agonist of intracellular store calcium channels.
Why This Matters
The study focused on markers of oxidative stress and calcium handling, described as two interrelated cellular processes previously shown to be affected by such radiation in other cell models. The researchers concluded that the data support a possible link between ELF-EMF exposure and modification of the cellular redox state, which could in turn increase the level of intracellular calcium.
Exposure Details
- Magnetic Field
- 0.1 and 1 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 5 min or 30 min
Exposure Context
This study used 0.1 and 1 mT for magnetic fields:
- 5Kx above the Building Biology guideline of 0.2 mG
- 1Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
Here, we applied a single-cell approach to study the effects of short-term exposure to extremely low frequency electromagnetic fields (ELF-EMFs) on muscle cell differentiation and function using C2C12 cells as an in vitro model of the skeletal muscle phenotype.
Our focus was on markers of oxidative stress and calcium (Ca2+) handling, two interrelated cellular ...
Collectively, our data reveal that ELF-EMFs (1) induced reactive oxygen species production in myobla...
In conclusion, our data support a possible link between exposure to ELF-EMFs and modification of the cellular redox state, which could, in turn, increase the level of intracellular Ca2+and thus modulate the metabolic activity of C2C12 cells.
Show BibTeX
@article{c_2010_modulation_of_redox_status_431,
author = {Morabito C and Rovetta F and Bizzarri M and Mazzoleni G and Fanò G and Mariggiò MA},
title = {Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach.},
year = {2010},
url = {https://www.sciencedirect.com/science/article/abs/pii/S089158490900759X},
}