Assessing the combined effect of extremely low-frequency magnetic field exposure and oxidative stress on LINE-1 promoter methylation in human neural cells.
Giorgi G, Pirazzini C, Bacalini MG, Giuliani C, Garagnani P, Capri M, Bersani F, Del Re B · 2017
View Original AbstractCombined exposure to a pulsed magnetic field and oxidative stress significantly decreased DNA methylation at different CpG sites in human neural cells, and most changes were transient.
Plain English Summary
Researchers exposed human neural cells (BE(2)C) to an extremely low frequency pulsed magnetic field (50 Hz, 1 mT) for 24 and 48 hours, alone and combined with oxidative stress, to study DNA methylation changes. Exposure to the magnetic field alone or oxidative stress alone produced only weak changes in DNA methylation levels at different CpG sites. When the two exposures were combined, a significant decrease in DNA methylation levels was found at different CpG sites, and most of those changes were transient.
Why This Matters
The study focused on LINE-1 elements, which are commonly used to evaluate the global genome methylation level. Methylation levels were measured at CpG sites located in the LINE-1 5' untranslated region using MassARRAY EpiTYPER. DNA methylation is described as one of the most often studied epigenetic modifications. The finding that most changes were transient suggests that cells can restore homeostatic DNA methylation patterns.
Exposure Details
- Magnetic Field
- 1 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 24 and 48 h
Exposure Context
This study used 1 mT for magnetic fields:
- 50Kx above the Building Biology guideline of 0.2 mG
- 10Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
In this paper, we investigated whether exposure to ELF-MF alone and in combination with oxidative stress (OS) can affect DNA methylation, which is one of the most often studied epigenetic modification.
To this end, we analyzed the DNA methylation levels of the 5'untranslated region (5'UTR) of long int...
The results indicate that exposures to the single agents PMF and OS induced weak decreases and incre...
The results are discussed and future research directions outlined.
Show BibTeX
@article{g_2017_assessing_the_combined_effect_375,
author = {Giorgi G and Pirazzini C and Bacalini MG and Giuliani C and Garagnani P and Capri M and Bersani F and Del Re B},
title = {Assessing the combined effect of extremely low-frequency magnetic field exposure and oxidative stress on LINE-1 promoter methylation in human neural cells.},
year = {2017},
url = {https://www.proquest.com/openview/50e7ace16d81cc67106070c97ce132dc/1?pq-origsite=gscholar&cbl=54037},
}