Power frequency magnetic fields affect the p38 MAPK-mediated regulation of NB69 cell proliferation implication of free radicals.
Martínez MA, Úbeda A, Moreno J, Trillo MÁ · 2016
View Original AbstractMagnetic field exposure promoted NB69 cell proliferation through p38 kinase activation, and this effect appeared to depend on reactive oxygen species.
Plain English Summary
Researchers investigated how power frequency magnetic fields affect cell proliferation in NB69 cells and the role of p38 and JNK kinases and reactive oxygen species in that response. NB69 cultures were exposed to a 100 µT magnetic field, either intermittently for 24, 42 or 63 hours, or continuously for periods of 15 to 120 minutes, with or without p38 or JNK inhibitors and an antioxidant. Field exposure induced transient activation of p38, JNK, and ERK1/2, but only the p38 inhibitor blocked the proliferative effect. The antioxidant NAC blocked the field effects on cell proliferation and p38 activation, but not those on ERK1/2 activation.
Why This Matters
To identify which signaling proteins were responsible, the study used specific inhibitors SB203580 and SP600125 to block p38 or JNK separately. Blocking p38 removed the proliferative effect, while blocking JNK did not, pointing to p38 as the key mediator. When the antioxidant NAC was used to neutralize reactive oxygen species, both the cell proliferation increase and p38 activation were blocked. The researchers concluded that the proliferative effects are likely mediated by a ROS-dependent activation of p38. The researchers described the proliferative effects as exerted through sequential upregulation of MAPK-p38 and ERK1/2 activation.
Exposure Details
- Magnetic Field
- 0.1 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 24, 42 or 63 h, or continuously for periods of 15 to 120 min
Exposure Context
This study used 0.1 mT for magnetic fields:
- 5Kx above the Building Biology guideline of 0.2 mG
- 1Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
This work investigates the MF effect on the cell cycle of NB69, the participation of p38 and c-Jun N-terminal (JNK) kinases in the field-induced proliferative response and the potential involvement of reactive oxygen species (ROS) in the activation of the MAPK-ERK1/2 and -p38 signaling pathways.
NB69 cultures were exposed to the 100 µT MF, either intermittently for 24, 42 or 63 h, or continuous...
Field exposure induced transient activation of p38, JNK and ERK1/2. The MF proliferative effect, whi...
Show BibTeX
@article{ma_2016_power_frequency_magnetic_fields_420,
author = {Martínez MA and Úbeda A and Moreno J and Trillo MÁ},
title = {Power frequency magnetic fields affect the p38 MAPK-mediated regulation of NB69 cell proliferation implication of free radicals.},
year = {2016},
url = {https://www.mdpi.com/1422-0067/17/4/510},
}