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Extremely low frequency electromagnetic field induces apoptosis of osteosarcoma cells via oxidative stress.

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Yang ML, Ye ZM · 2015

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Exposing human osteosarcoma MG-63 cells to a 50 Hz, 1 mT extremely low frequency electromagnetic field induced cell apoptosis and increased reactive oxygen species.

Plain English Summary

Summary written for general audiences

Researchers exposed human osteosarcoma MG-63 cells to a 50 Hz, 1 mT extremely low frequency electromagnetic field (ELF-EMF) for 1, 2 and 3 hours in vitro. ELF-EMF decreased the viability of MG-63 cells, inhibited cell growth, induced cell apoptosis and increased the level of ROS significantly. The apoptosis rate declined significantly after treatment with ROS inhibitor NAC or p38MAPK inhibitor SB203580. The researchers concluded that increased ROS and p38MAPK activation may be involved in the mechanism.

Why This Matters

The study used MG-63 cells, a human osteosarcoma cell line, exposed in vitro to ELF-EMF at 50 Hz and 1 mT. Cell proliferation was measured by the MTT method, while the apoptosis rate and ROS levels were detected by flow cytometry. After exposure to ELF-EMF, p38MAPK in MG-63 cells was activated, and the phosphorylation level was also inhibited after treatment with NAC. Some cells were pretreated with either the ROS inhibitor NAC or the p38MAPK inhibitor SB203580 to help identify the pathway involved.

Exposure Details

Magnetic Field
1 mT
Source/Device
50 Hz
Exposure Duration
1, 2 and 3 h

Exposure Context

This study used 1 mT for magnetic fields:

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Exposure Level in ContextStudy Exposure Level in ContextThis study: 1 mTExtreme Concern - 5 mGFCC Limit - 2,000 mGEffects observed in the Extreme Concern range (Building Biology)This level is 5 times the FCC limit
A logarithmic frequency spectrum from 10 Hz to 100 GHz showing where this study's 50 Hz exposure sits relative to common EMF sources.Where This Frequency Sits on the EMF SpectrumELFVLFLF / MFHF / VHFUHFSHFmm10 Hz100 GHzThis study: 50 HzCell phones~1 GHzWiFi2.4 GHz5G mm28 GHzLogarithmic scale

Study Details

To investigate the effects of extremely low frequency electromagnetic field (ELF-EMF) on human osteosarcoma cells and its mechanisms.

Human osteosarcoma MG-63 cells were exposed to 50 Hz,1 mT ELF-EMF for 1, 2 and 3 h in vitro, with o...

ELF-EMF decreased the viability of MG-63 cells, inhibited cell growth, induced cell apoptosis and in...

ELF-EMF can induce the apoptosis of MG-63 cells. Increased ROS and p38MAPK activation may be involved in the mechanism.

Cite This Study
Yang ML, Ye ZM (2015). Extremely low frequency electromagnetic field induces apoptosis of osteosarcoma cells via oxidative stress. Zhejiang Da Xue Xue Bao Yi Xue Ban. 44(3):323-328, 2015.
Show BibTeX
@article{ml_2015_extremely_low_frequency_electromagnetic_482,
  author = {Yang ML and Ye ZM},
  title = {Extremely low frequency electromagnetic field induces apoptosis of osteosarcoma cells via oxidative stress.},
  year = {2015},
  
  url = {https://pubmed.ncbi.nlm.nih.gov/26350014/},
}
PubMed: 26350014

Cited By (15 papers)

Quick Questions About This Study

The study used human osteosarcoma MG-63 cells exposed in vitro.
Cells were exposed to 50 Hz, 1 mT ELF-EMF for 1, 2 and 3 hours in vitro.
ELF-EMF induced cell apoptosis and decreased the viability of MG-63 cells.
The apoptosis rate declined significantly after treatment with ROS inhibitor NAC.
The researchers suggested that increased ROS and p38MAPK activation may be involved in the mechanism.