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Oxidative Stress129 citations

The phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field.

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Roy S, Noda Y, Eckert V, Traber MG, Mori A, Liburdy R, Packer L · 1995

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Magnetic fields at household appliance levels increased harmful free radical production in immune cells by 12.4%.

Plain English Summary

Summary written for general audiences

Researchers exposed rat immune cells called neutrophils to a weak 60 Hz magnetic field (0.1 mT) and found it increased their production of free radicals by 12.4% when the cells were stimulated. Free radicals are reactive molecules that can damage cells and contribute to inflammation and disease. This was the first study to show that magnetic fields can directly influence free radical production in living immune cells.

Why This Matters

This research breaks important ground by demonstrating that extremely low frequency magnetic fields can amplify oxidative stress in immune cells. The 0.1 mT exposure level is significant because it falls within the range you might encounter near household electrical wiring or appliances, though typically for much shorter durations than laboratory exposure. What makes this study particularly noteworthy is that it shows magnetic fields don't just affect cells passively, but can actually enhance the cellular processes that generate harmful free radicals. The 12.4% increase in oxidative burst may seem modest, but when you consider that chronic low-level inflammation contributes to numerous health conditions, even small increases in free radical production could have meaningful biological consequences over time.

Exposure Details

Magnetic Field
0.1 mG
Source/Device
60 Hz

Exposure Context

This study used 0.1 mG for magnetic fields:

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Exposure Level in ContextA logarithmic scale showing exposure levels relative to Building Biology concern thresholds and regulatory limits.Study Exposure Level in ContextThis study: 0.1 mGExtreme Concern5 mGFCC Limit2,000 mGEffects observed in the No Concern range (Building Biology)FCC limit is 20,000x higher than this exposure level

Study Details

We have investigated whether low frequency (60 Hz) low intensity (0.1 mT) MF can modulate the phorbol 12-myristate 13- acetate (PMA) induced respiratory burst in primed rat peritoneal neutrophils, followed in real time using the dye 2′,7′-dichlorofluorescin (DCFH), which reacts with free radical-derived oxidants such as H2O2 (which is formed from the dismutation of superoxide) to become 2′,7′-dichlorofluorecein (DCF), a highly fluorescent compound.

In the presence of the MF, a 12.4% increase in the fluorescence signal was observed in PMA-stimulate...

We believe this represents the first experimental observation of MF influencing events involving free radical species generated during signal transduction in living cells.

Cite This Study
Roy S, Noda Y, Eckert V, Traber MG, Mori A, Liburdy R, Packer L (1995). The phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field. FEBS Lett. 376(3):164-166, 1995.
Show BibTeX
@article{s_1995_the_phorbol_12myristate_13acetate_453,
  author = {Roy S and Noda Y and Eckert V and Traber MG and Mori A and Liburdy R and Packer L},
  title = {The phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field.},
  year = {1995},
  doi = {10.1016/0014-5793(95)01266-X},
  url = {https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793(95)01266-X},
}

Quick Questions About This Study

Researchers exposed rat immune cells called neutrophils to a weak 60 Hz magnetic field (0.1 mT) and found it increased their production of free radicals by 12.4% when the cells were stimulated. Free radicals are reactive molecules that can damage cells and contribute to inflammation and disease. This was the first study to show that magnetic fields can directly influence free radical production in living immune cells.