Electromagnetic field could protect SH-SY5Y cells against cisplatin cytotoxicity, but not MCF-7 cells.
Mahmoudinasab H, Saadat M. · 2018
View Original AbstractElectromagnetic field exposure combined with cisplatin reduced the drug's cytotoxicity in SH-SY5Y cells but produced no significant change in MCF-7 cells.
Plain English Summary
Researchers tested four treatment combinations on MCF-7 and SH-SY5Y cells: cisplatin alone, cisplatin with morin, cisplatin with electromagnetic field, and cisplatin combined with both morin and electromagnetic field. The electromagnetic field was applied at 50 Hz and 0.50 mT using a cycle of 15 minutes field-on followed by 15 minutes field-off. The IC50 value of cisplatin in the cisplatin-plus-morin-plus-EMF treatment was significantly higher than cisplatin alone and the cisplatin-plus-morin treatment in both cell lines. In SH-SY5Y cells, most of the antioxidant genes showed upregulation with the cisplatin-plus-EMF and cisplatin-plus-morin-plus-EMF treatments, while antioxidant gene expression patterns were similar across the four treatments in MCF-7 cells.
Why This Matters
The researchers also measured the mRNA expression levels of 11 antioxidant genes across both cell lines and all four treatments. Significant differences in the number of upregulated genes were observed between different treatments in SH-SY5Y cells. The researchers suggested that the molecular mechanism behind reduced cisplatin cytotoxicity in their combinations is probably different in MCF-7 and SH-SY5Y cells.
Exposure Details
- Magnetic Field
- 0.5 mT
- Source/Device
- 50 Hz
- Exposure Duration
- 15 min field-on/15 min field-off
Exposure Context
This study used 0.5 mT for magnetic fields:
- 25Kx above the Building Biology guideline of 0.2 mG
- 5Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
In this study, we tried to increase the cytotoxicity of CDDP in combination with Mor and/or EMF in MCF-7 and SH-SY5Y cells. Furthermore, we evaluate the expression levels of 11 antioxidant genes in both cell lines.
We designed four treatments: CDDP alone, “CDDP+Mor,” “CDDP+EMF,” and “CDDP+Mor+EMF.” Serial dilution...
The IC50 value of CDDP in “CDDP+Mor+EMF” treatment was significantly higher than CDDP alone and “CDD...
Show BibTeX
@article{h_2018_electromagnetic_field_could_protect_415,
author = {Mahmoudinasab H and Saadat M.},
title = {Electromagnetic field could protect SH-SY5Y cells against cisplatin cytotoxicity, but not MCF-7 cells. },
year = {2018},
doi = {10.1089/dna.2017.4108},
url = {https://www.liebertpub.com/doi/abs/10.1089/dna.2017.4108},
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