Electromagnetic fields induce neural differentiation of human bone marrow derived mesenchymal stem cells via ROS mediated EGFR activation.
Park JE, Seo YK, Yoon HH, Kim CW, Park JK, Jeon S · 2013
View Original AbstractExposure to ELF-EMF (50 Hz, 1 mT) induced neural differentiation of human bone marrow mesenchymal stem cells, apparently through ROS generation and EGFR signaling.
Plain English Summary
Researchers treated human bone marrow derived mesenchymal stem cells (hBM-MSCs) with ELF-EMF at 50 Hz and 1 mT and measured neural marker expression and cell signaling. The expression of neural markers NF-L, MAP2, and NeuroD1 increased at 6 days, while phosphorylation of Akt and CREB, but not ERK, increased at 90 min. The researchers suggest that EMF induces neural differentiation through activation of EGFR signaling and mild generation of reactive oxygen species (ROS).
Why This Matters
To probe the signaling pathway, the researchers examined CREB phosphorylation and activation of Akt or ERK as upstream molecules of CREB. EMF also increased phosphorylation of the epidermal growth factor receptor (EGFR), described as an upstream receptor tyrosine kinase of PI3K/Akt, at 90 min. It has been well documented that ELF-MF exposure may alter cellular processes by increasing intracellular reactive oxygen species (ROS) concentrations, and the researchers examined EMF-induced ROS production in hBM-MSCs. Pretreatment with a ROS scavenger (N-acetylcystein) and an EGFR inhibitor (AG-1478) prevented phosphorylation of EGFR and downstream molecules, supporting a role for ROS and EGFR in the observed effects.
Exposure Details
- Magnetic Field
- 1 mT
- Source/Device
- 50 Hz
Exposure Context
This study used 1 mT for magnetic fields:
- 50Kx above the Building Biology guideline of 0.2 mG
- 10Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
To find out the signaling pathways involved in the neural differentiation of BM-MSCs by EMF, we examined the CREB phosphorylation and Akt or ERK activation as an upstream of CREB.
In hBM-MSCs treated with ELF-EMF (50 Hz, 1 mT), the expression of neural markers such as NF-L, MAP2...
These results suggest that EMF induce neural differentiation through activation of EGFR signaling and mild generation of ROS.
Show BibTeX
@article{je_2013_electromagnetic_fields_induce_neural_438,
author = {Park JE and Seo YK and Yoon HH and Kim CW and Park JK and Jeon S},
title = {Electromagnetic fields induce neural differentiation of human bone marrow derived mesenchymal stem cells via ROS mediated EGFR activation.},
year = {2013},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0197018613000417},
}