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Effect of 50-Hz sinusoidal magnetic field on the production of superoxide anion and the expression of heat-shock protein 70 in RAW264 cells

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Pooam M, Nakayama M, Nishigaki C, Miyata H · 2017

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Power frequency magnetic fields at 0.1-0.5 milliTesla damage cellular energy production and trigger stress responses in immune cells.

Plain English Summary

Summary written for general audiences

Scientists exposed immune cells to 50 Hz magnetic fields from power lines at levels found near electrical devices. The magnetic fields damaged cellular energy centers, increased harmful free radicals, and triggered stress responses. This suggests everyday magnetic field exposure may stress our immune systems.

Why This Matters

This study adds important evidence to our understanding of how extremely low frequency magnetic fields affect cellular health at the most fundamental level. The researchers found clear biological effects at magnetic field strengths of 0.1 to 0.5 milliTesla, which are within the range you might encounter near power lines, electrical panels, or some household appliances. What makes this research particularly significant is that it shows a clear mechanism of harm: the magnetic fields disrupt mitochondrial function, which then triggers a cascade of cellular stress responses including free radical production and heat shock protein expression. These are the same stress markers your cells produce when exposed to toxins or other harmful substances. The reality is that your immune cells are constantly working to protect you, and this study demonstrates that magnetic field exposure may be forcing them to work harder by creating additional oxidative stress. While more research is needed to understand long-term health implications, the evidence shows these fields are not biologically neutral as often claimed.

Exposure Details

Magnetic Field
0.1 or 0.5 mG
Source/Device
50 Hz

Exposure Context

This study used 0.1 or 0.5 mG for magnetic fields:

Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.

Where This Falls on the Concern Scale

Study Exposure Level in ContextA logarithmic scale showing exposure levels relative to Building Biology concern thresholds and regulatory limits.Study Exposure Level in ContextThis study: 0.1 or 0.5 mGExtreme Concern5 mGFCC Limit2,000 mGEffects observed in the No Concern range (Building Biology)FCC limit is 20,000x higher than this exposure level

Study Details

we have investigated the effect of 50-Hz sinusoidal magnetic field on the production of O2-, the expression of heat shock protein (HSP) 70 and the mitochondrial membrane potential in cell line macrophage RAW264 cells.

Macrophages were exposed to or not exposed to 0.1-mT or 0.5-mT, 50-Hz sinusoidal magnetic field and ...

The 50-Hz magnetic field enhanced production of O2- and the expression of HSP70, both of which are c...

The results collectively suggest that the 50-Hz magnetic field diminished the mitochondrial membrane potential, which led to the increase in the production of O2- and the expression of HSP70 protein

Cite This Study
Pooam M, Nakayama M, Nishigaki C, Miyata H (2017). Effect of 50-Hz sinusoidal magnetic field on the production of superoxide anion and the expression of heat-shock protein 70 in RAW264 cells Int J Chem 9:23-36, 2017.
Show BibTeX
@article{m_2017_effect_of_50hz_sinusoidal_445,
  author = {Pooam M and Nakayama M and Nishigaki C and Miyata H},
  title = {Effect of 50-Hz sinusoidal magnetic field on the production of superoxide anion and the expression of heat-shock protein 70 in RAW264 cells},
  year = {2017},
  
  url = {https://pdfs.semanticscholar.org/4c4a/b98b06feaa5915578aa574b7fe47e4f887d7.pdf},
}

Quick Questions About This Study

Scientists exposed immune cells to 50 Hz magnetic fields from power lines at levels found near electrical devices. The magnetic fields damaged cellular energy centers, increased harmful free radicals, and triggered stress responses. This suggests everyday magnetic field exposure may stress our immune systems.