Effect of 50-Hz sinusoidal magnetic field on the production of superoxide anion and the expression of heat-shock protein 70 in RAW264 cells
Pooam M, Nakayama M, Nishigaki C, Miyata H · 2017
View Original AbstractExposing RAW264 macrophage cells to a 50-Hz magnetic field increased superoxide production and HSP70 expression while reducing mitochondrial membrane potential.
Plain English Summary
Researchers exposed RAW264 macrophage cells to a 50-Hz sinusoidal magnetic field at 0.1 mT or 0.5 mT and measured superoxide production, HSP70 expression, and mitochondrial membrane potential. The magnetic field enhanced production of superoxide and the expression of HSP70. The researchers suggested that the 50-Hz magnetic field diminished mitochondrial membrane potential, which led to increased superoxide production and HSP70 expression.
Why This Matters
The experiments were conducted in cell line macrophage RAW264 cells. Field strengths of 0.1 mT and 0.5 mT were tested at 50 Hz. Mitochondrial membrane potential was tracked using a fluorescent indicator. As a comparison, the researchers also applied carbonyl cyanide p-trifluoromethoxyphenylhydrazone, a known uncoupler of mitochondrial function, which both reduced membrane potential and increased superoxide production. The researchers noted that the enhanced superoxide production and HSP70 expression they observed were consistent with previous studies.
Exposure Details
- Magnetic Field
- 0.1 or 0.5 mT
- Source/Device
- 50 Hz
Exposure Context
This study used 0.1 or 0.5 mT for magnetic fields:
- 5Kx above the Building Biology guideline of 0.2 mG
- 1Kx above the BioInitiative Report recommendation of 1 mG
Building Biology guidelines are practitioner-based limits from real-world assessments. BioInitiative Report recommendations are based on peer-reviewed science. Check Your Exposure to compare your own measurements.
Where This Falls on the Concern Scale
Study Details
we have investigated the effect of 50-Hz sinusoidal magnetic field on the production of O2-, the expression of heat shock protein (HSP) 70 and the mitochondrial membrane potential in cell line macrophage RAW264 cells.
Macrophages were exposed to or not exposed to 0.1-mT or 0.5-mT, 50-Hz sinusoidal magnetic field and ...
The 50-Hz magnetic field enhanced production of O2- and the expression of HSP70, both of which are c...
The results collectively suggest that the 50-Hz magnetic field diminished the mitochondrial membrane potential, which led to the increase in the production of O2- and the expression of HSP70 protein
Show BibTeX
@article{m_2017_effect_of_50hz_sinusoidal_445,
author = {Pooam M and Nakayama M and Nishigaki C and Miyata H},
title = {Effect of 50-Hz sinusoidal magnetic field on the production of superoxide anion and the expression of heat-shock protein 70 in RAW264 cells},
year = {2017},
url = {https://pdfs.semanticscholar.org/4c4a/b98b06feaa5915578aa574b7fe47e4f887d7.pdf},
}