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Effects of 700 and 3500 MHz 5G radiofrequency exposure on developing zebrafish embryos

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Torres-Ruiz M, Suárez OJ, López V, Marina P, Sanchis A, Liste I, de Alba M, Ramos V · 2024

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Zebrafish embryos exposed to unmodulated 700 MHz and 3500 MHz radiation showed no changes in mortality, hatching, or body length, but had organ morphological changes, behavioral alterations, and stronger effects at 700 MHz with 4 h exposure.

Plain English Summary

Summary written for general audiences

Researchers exposed zebrafish embryos to unmodulated 700 MHz and 3500 MHz radiofrequency radiation for 1 and 4 hours during the blastula period and assessed outcomes up to 120 hours post fertilization. The exposure did not affect mortality, hatching, or body length, but caused specific organ morphological changes and lasting behavioral alterations in activity, anxiety-like behavior, and habituation. Effects were stronger at 700 MHz, particularly after 4 hours of exposure, and acetylcholinesterase activity was reduced.

Why This Matters

The study employed a battery of toxicity, developmental, and behavioral assays, including tail coiling, light/dark activity, thigmotaxis anxiety, and startle response habituation tests. The exposure system was described as robust, flexible for frequency and power testing, and highly comparable. The authors plan future experiments with modulated 5G signals to further assess health effects.

Exposure Information

A logarithmic frequency spectrum from 10 Hz to 100 GHz showing where this study's 700 MHz, 3500 MHz exposure sits relative to common EMF sources.Where This Frequency Sits on the EMF SpectrumELFVLFLF / MFHF / VHFUHFSHFmm10 Hz100 GHzThis study: 700 MHz, 3500 MHzPower lines50/60 HzWiFi2.4 GHz5G mm28 GHzLogarithmic scale

Specific exposure levels were not quantified in this study.

Cite This Study
Torres-Ruiz M, Suárez OJ, López V, Marina P, Sanchis A, Liste I, de Alba M, Ramos V (2024). Effects of 700 and 3500 MHz 5G radiofrequency exposure on developing zebrafish embryos.
Show BibTeX
@article{torres_ruiz_m_surez_oj_lpez_v_marina_p_sanchis_a_liste_i_de_alba_m_ramos_v_ce3887,
  author = {Torres-Ruiz M and Suárez OJ and López V and Marina P and Sanchis A and Liste I and de Alba M and Ramos V},
  title = {Effects of 700 and 3500 MHz 5G radiofrequency exposure on developing zebrafish embryos},
  year = {2024},
  doi = {10.1016/j.scitotenv.2023.169475},
  
}

Quick Questions About This Study

The study used unmodulated 700 MHz and 3500 MHz frequencies with exposure durations of 1 hour and 4 hours during the blastula period.
No effects on mortality, hatching, or body length were observed.
Exposed larvae showed alterations in activity, anxiety-like behavior, and habituation that persisted after early embryonic exposure.
Effects were more pronounced at 700 MHz, especially after 4 hours of exposure.
Acetylcholinesterase activity decreased in exposed embryos.