Unknown authors · 2021
Researchers exposed peripheral blood cells from five male donors to a continuous 900 MHz electromagnetic field for up to 90 minutes. They measured expression of 667 microRNAs using a quantitative PCR array and compared the results to sham and hyperthermia controls. In the initial screening, two miRNAs — miR-194 at 30 minutes and miR-939 at 60 minutes — were down-regulated about two-fold and their p-values survived Bonferroni correction. A repeat experiment two years later found similar overall miRNA expression patterns but could not reproduce the previously identified differentially expressed miRNAs, and the authors concluded that the data do not support early miRNA changes or EMF-responsive individuals.
Unknown authors · 2021
Researchers exposed pregnant mice to 900 MHz cell phone radiation for 8 hours daily over 10 days and found significant increases in oxidative stress markers in their blood. The study showed that vitamin C supplementation could prevent this radiation-induced cellular damage, suggesting antioxidants may offer protection against EMF exposure during pregnancy.
Unknown authors · 2021
Researchers exposed human A172 glioblastoma cells to 1800 MHz continuous-wave or GSM-modulated RF fields at 2.0 W/kg for 5, 30, or 240 minutes with or without serum. They measured phosphorylated and total signal transduction proteins and antioxidant proteins using multiplex bead arrays. In serum-containing cultures, continuous-wave exposure decreased CAT expression after 30 minutes, total JNK at 30 and 240 minutes, STAT3 after 240 minutes, and phosphorylated CREB after 30 minutes. In serum-free cultures, GSM-modulated exposure decreased total STAT5 after 30 and 240 minutes. The authors concluded that the observed changes were sporadic across conditions and likely false positives, with no consistent evidence of changes in antioxidant or signal transduction proteins.
Unknown authors · 2021
The study found that exposure to RFID signals at a specific absorption rate of 2 W/kg is sufficient to alter serotonin metabolism in rats. This effect occurred regardless of whether the exposure level was considered biohazardous.
Unknown authors · 2020
Researchers exposed young adult male Wistar rats to mobile phone radiofrequency electromagnetic field radiation at 1966.1 MHz, 4.0 mW/cm2, and 0.36 W/kg for 2 hours per day over 16 weeks. Another set of animals was kept under similar conditions without radiation exposure as controls. At the end of the exposure period, the rats were tested for fear memory and then euthanized to measure hippocampal oxidative stress, circulating pro-inflammatory cytokines, and stress hormones. Exposed rats showed significantly increased hippocampal oxidative stress, elevated levels of IL-1beta, IL-6, and TNF-alpha, increased adrenal gland weight, and higher levels of ACTH and corticosterone. However, changes in contextual fear memory were not statistically significant.
Unknown authors · 2020
Researchers exposed rats to mobile phone radiation (1966.1 MHz) for 2 hours daily over 16 weeks and found significant increases in brain oxidative stress, inflammatory markers, and stress hormones. The study demonstrates that chronic cell phone radiation exposure can trigger biological stress responses in the brain and body, even at levels similar to everyday phone use.
Unknown authors · 2020
Researchers exposed N9 microglial and SH-SY5Y neuroblastoma cells to ELF-modulated 935 MHz RF-EMF at 4 W/kg for 2 and 24 hours. Exposure for 24 hours increased the autophagy marker ATG5, but did not change LC3B-I, LC3B-II, or pERK in either cell type. A transient increase in glutathione was observed only in SH-SY5Y cells, while hydrogen peroxide and cytochrome c oxidase were unaffected. Apoptosis, living cell number, and apoptosis-inducing factor were not altered by exposure at either duration.
Gulati et al. · 2020
Researchers compared genotoxic effects of three UMTS frequency channels used by 3G phones (1923, 1947.47, and 1977 MHz) in human lymphocytes. Human lymphocytes were exposed for 1 hour and 3 hours and tested for ROS, DNA damage, TP53 mutations, preleukemic fusion genes, and apoptosis. No effects were seen on ROS, apoptosis, preleukemic fusion genes, or TP53 mutations at any frequency, though bulk RNA expression was inhibited. A small but statistically significant increase in DNA damage occurred depending on the UMTS frequency channel, with the maximum effect at 1977.0 MHz.
Unknown authors · 2020
The study used 32 adult male BALB/c mice divided into four groups: control, melatonin (2 mg/kg intraperitoneally for 30 consecutive days), RF-EMR exposure (900 MHz, 100 to 300 MT, 54 to 160 W/m, 4 hours per day whole body), and melatonin plus RF-EMR. After exposure, researchers evaluated oxidative stress parameters, testosterone level, and testicular histopathology. RF-EMR exposure induced oxidative stress, testicular tissue damage, and decreased testosterone. Melatonin treatment improved oxidative stress parameters (MDA and GSH), testis injury score, increased germinal epithelial thickness and seminiferous tubule diameter, and decreased testosterone in EMR-exposed mice, with significant differences (p < .05).
Unknown authors · 2020
The study aimed to investigate the effects of 900 MHz EMF exposure (60 min/day for 28 days) on rat testis and to determine whether thymoquinone (10 mg/kg/day) reduced the deleterious effect. EMF exposure significantly decreased primary spermatocytes, spermatids, Leydig cells, serum testosterone, and testis weight compared to controls. Thymoquinone treatment significantly increased primary spermatocyte numbers compared to EMF alone.
Gulati et al. · 2020
Researchers exposed human immune cells to three different 3G cell phone frequencies (1923, 1947, and 1977 MHz) for 1-3 hours to test for DNA damage and cellular stress. They found small but significant DNA damage that varied by frequency, with 1977 MHz causing the most harm, while other cellular damage markers remained unchanged.
Unknown authors · 2020
Researchers exposed male mice to 900 MHz mobile phone radiation for 4 hours daily and found it caused testicular damage, oxidative stress, and decreased testosterone levels. However, when mice were also given melatonin supplements, the hormone significantly reduced these harmful effects. This suggests melatonin's antioxidant properties may help protect reproductive health from cell phone radiation exposure.
Azimzadeh M, Jelodar G · 2020
The study examined the effects of 900 MHz RFW on brain iron, copper, zinc, and manganese concentrations and the protective role of vitamin E. Twenty adult male Sprague-Dawley rats were divided into four groups: control, vitamin E only, vitamin E plus RFW exposure, and RFW exposure only. The rats were exposed to RFW for 30 consecutive days at 4 hours per day while freely moving in the cage. Brain tissue levels of the elements were measured on the last day using atomic absorption spectrophotometry. Exposure significantly increased iron, copper, manganese, and the copper-to-zinc ratio while decreasing zinc, and vitamin E pre-treatment improved iron, copper, manganese, and the ratio but not zinc.
Azimzadeh M, Jelodar G · 2020
This study examined the effects of 900 MHz radiofrequency exposure on trace element concentrations (iron, copper, zinc, and manganese) in rat brain tissue, and investigated whether vitamin E pretreatment could provide protective effects. The results showed that radiofrequency exposure disrupted trace element homeostasis by increasing iron, copper, and manganese levels while decreasing zinc, and vitamin E supplementation partially mitigated these changes.
Azimzadeh M et al. · 2020
Researchers exposed pregnant Sprague-Dawley rats and their pups to 900 MHz radiofrequency waves from a base transceiver station antenna during gestation or the first 21 days after birth for 2 h/d or 4 h/d. Passive avoidance learning and memory were assessed in 45-day-old pups with a shuttle box, and hippocampal histomorphology was examined. All exposed subgroups showed impaired passive avoidance learning and memory, the prenatal exposure group had the greatest deficit, and a mild decrease in pyramidal cell density was seen in exposed subgroups I and III.
Azimzadeh M, Jelodar G · 2020
Researchers exposed pregnant rats and newborn rat pups to 900 MHz radiofrequency radiation (similar to cell phone towers) and tested their learning and memory abilities at 45 days old. All exposed groups showed impaired learning and memory performance, with prenatal exposure causing the most severe effects. Brain tissue examination revealed decreased nerve cell density in memory-related brain regions.
Unknown authors · 2020
Vafaei S, Motejaded F, Ebrahimzadeh-Bideskan A · 2020
Unknown authors · 2020
Jeong et al. · 2020
Public concerns exist about deleterious biological consequences of radiofrequency electromagnetic fields. These concerns have led to wide consideration of the potential effects of RF-EMF on the central nervous system.
Jeong et al. · 2020
Researchers exposed mice to 1,950 MHz radiofrequency radiation (similar to cell phone frequencies) for an extended period and observed behavioral changes along with alterations in gene expression patterns. The study focused on potential effects to the central nervous system, finding measurable impacts on both mouse behavior and genetic activity. This adds to growing evidence that chronic RF exposure may influence brain function and cellular processes.
Unknown authors · 2020
Pregnant rats were exposed to radiofrequency fields starting on day 21 of pregnancy, and offspring were tested with Y maze at 3 weeks, open field at 7 weeks, and hippocampal NMDAR expression at 9 weeks. The 1800 MHz and 1800 MHz + WiFi groups showed a downward trend in body weight, and the WiFi group opened eyes earlier than controls. In open field tests, the 1800 MHz + WiFi group had shorter overall path length and delayed stationary time, the WiFi group had shorter path length and increased velocity in the error arm, and the 1800 MHz + WiFi group showed increased central area activity. Both exposure groups down-regulated NR2A and NR2B and up-regulated NR2D, NR3A, and NR3B, while the WiFi group also up-regulated NR1 and NR2C.
Unknown authors · 2020
Researchers exposed pregnant rats to 1800 MHz cell phone radiation and 2400 MHz WiFi signals, then tested their offspring's behavior and brain development. The exposed pups showed altered movement patterns, changed brain chemistry, and different developmental timing compared to unexposed controls. The study suggests prenatal EMF exposure may affect how the brain develops during critical early periods.
Unknown authors · 2020
Researchers applied a 2-h head-only GSM-1800 MHz exposure to rats with LPS-induced acute neuroinflammation, to healthy rats, and to transgenic hSOD1 G93A rats modeling presymptomatic ALS. Gene responses were measured 24 hours later in a motor area of the cerebral cortex where the mean SAR was 3.22 W/kg. In LPS-treated rats, RNA-seq revealed significant but moderate gene expression changes affecting 2.7% of expressed genes, including neuronal and glial genes and genes involved in protein ubiquitination or dephosphorylation.
Hepatic injury induced by radio frequency waves emitted from conventional Wi-Fi devices in Wistar ratsH M Fahmy et al. · 2020
Researchers exposed Wistar female rats to a standard 2.45 GHz radio frequency source with an averaged whole-body specific absorption rate of 0.01 W kg−1 24 h−1 daily for 40 consecutive days. The rats were randomly divided into control and Wi-Fi–exposed groups. Liver samples were evaluated for oxidative stress parameters, transaminase levels, molecular structure using Fourier transform infrared spectroscopy, histopathology, and ultrastructure. Exposure led to severe oxidative stress, deleterious effects on liver function, altered molecular structure, and severe histological and ultrastructural alterations indicating hepatotoxic effects.
