Bektas H, Yildirim S, Cakir S, Dogu S, Altindag F · 2026
Twenty-eight adult male Wistar rats were divided into Control, RF, CoQ10, and RF + CoQ10 groups. RF exposure lowered testosterone, LH, and FSH, raised MDA and TOS, and produced degenerative testicular changes; CoQ10 partially reversed these changes by restoring testosterone and TAS and reducing tissue damage.
Bektas H, Seker A, Ustun R, Dogu S · 2026
Researchers exposed primary mouse dorsal root ganglion cultures to pulsed 3.5 GHz GSM-like RF-EMF for 1 to 24 hours in a GTEM-based setup. Dosimetry confirmed non-thermal exposure with temperature differences below 0.1°C. They assessed cell viability, ROS levels, mitochondrial apoptotic markers (Bax, Bcl-2, cytochrome c, caspase-3), and p75^NTR using blinded confocal analysis. Exposure caused a time-dependent reduction in viability, robust ROS elevations, increased Bax and caspase-3, decreased Bcl-2, and cytochrome c release, with maximal effects at 12 to 24 hours. p75^NTR upregulation indicated maladaptive neurotrophin signaling.
Effects of wireless local area network exposure on testicular morphology and VEGF levels Çakmak E et al. · 2026
VEGFA gene expression and protein levels increased significantly in the exposed group while HIF1A gene expression did not change. Exposed rats had reduced seminiferous tubule diameter, epithelial thickness, tubule density, and Sertoli cell count.
Kim JH et al. · 2026
Researchers exposed human keratinocytes and murine melanoma cells to UVA radiation followed by 5G RF-EMFs at 3.5 and 28 GHz. Post-UV RF-EMF exposure significantly reduced DNA damage markers such as γH2AX foci formation by approximately 30–50% and comet tail moments by 60–80%, and suppressed intracellular ROS accumulation by 56–93%. These effects were accompanied by selective attenuation of p38 MAPK phosphorylation reduced by 55–85%. The magnitude of molecular protection was comparable to that of N-acetylcysteine treatment or pharmacological inhibition of p38 MAPK. However, RF-EMF exposure did not reverse UV-induced reductions in cell viability or alterations in cell cycle distribution.
Unknown authors · 2025
Chick embryos were exposed continuously to 2.4 GHz EMR for 4 hours each day over 5 days, and SH-SY5Y cells were also exposed to assess cell viability, DNA damage, and apoptosis. Exposure increased ROS production in SH-SY5Y cells after 4 hours, caused moderate DNA damage, and upregulated the Bax gene as an early apoptosis marker. Antioxidants NAC and Mito-TEMPO reduced the cytotoxic effects of EMR in both models.
Unknown authors · 2025
Researchers exposed developing chick embryos and human nerve cells to 2.4 GHz radiation (the same frequency used by WiFi and Bluetooth) for 4 hours daily over 5 days. They found increased oxidative stress and early signs of cell death in both models, though antioxidants helped reduce these harmful effects. The study suggests even short-term exposure to common wireless frequencies can damage developing brain tissue at the cellular level.
Unknown authors · 2025
Researchers exposed developing chick embryos and human brain cells to 2.4 GHz radiation (the same frequency used by WiFi and Bluetooth) for 4 hours daily over 5 days. The study found that this short-term exposure increased oxidative stress and triggered early signs of cell death in brain tissue, though antioxidants helped reduce these harmful effects.
Bektas H, Bese Akgun BB, Cakir S, Dogu S, Ahnas B · 2025
Unknown authors · 2025
Researchers exposed human fibroblasts to 5G RF-EMF at SAR of 0.08 and 4 W/kg for 24 hours using BRET-based biosensors. No significant effect was found on oxidative stress markers, adaptive response, or CPD repair kinetics and efficiency.
Miles A, Porch A, Choi H, Cripps S, Brown H, Williams C · 2025
Researchers designed a high-throughput waveguide applicator that exposed 96 samples simultaneously to a quantifiable electric field at 2.45 GHz. Staphylococcus aureus bacteria received non-thermal microsecond-pulsed microwave exposure at 25 W r.m.s. for 24 h. After exposure, the bacteria showed significantly higher optical density measurements and growth rates than thermal controls. Fluorescent probes indicated that microwave exposure altered the cellular redox state.
Unknown authors · 2025
The study applied continuous Wi-Fi radiation at 2.4 GHz and 200–500 µW/m² for nine days to chicken embryos. Overall mesonephros development was not adversely affected, but moderate diffuse degenerative changes appeared in mesonephric corpuscles and tubules. Congested blood vessels were observed in the interstitium without inflammatory infiltrate. The exposed group showed significantly more apoptotic and proliferating cells and up-regulated caspase-1 gene expression.
Unknown authors · 2025
Researchers exposed human skin cells to 5G radiofrequency fields at 3.5 GHz for 24 hours to test for cellular damage. They found no increase in oxidative stress or DNA repair problems, even at exposure levels 50 times higher than typical phone use. The study suggests 5G frequencies may not harm skin cells under laboratory conditions.
Unknown authors · 2025
Researchers exposed mice to a 5G-modulated 3.5 GHz signal for 1 hour daily, 5 days per week over six weeks at a brain-averaged SAR of 0.19 W/kg. Behavioral tests after four weeks showed no significant effects on locomotion, anxiety, or memory. mRNA profiling at the end revealed significant expression changes in less than 1% of genes, with over-representation of glutamatergic synapse genes. The right cortical area with a SAR of 0.43 W/kg showed more mitochondrial genome-encoded responsive genes than the left area with a SAR of 0.14 W/kg.
Unknown authors · 2025
Researchers exposed mice to 5G signals at 3.5 GHz for six weeks, finding no changes in behavior, memory, or anxiety levels. However, the radiation did alter gene expression in brain cells, particularly affecting genes related to brain communication pathways. The study shows 5G can cause biological changes even when behavioral effects aren't obvious.
Unknown authors · 2025
Swiss researchers exposed 34 people to 5G signals (3.6 GHz and 700 MHz) for 30 minutes before sleep and monitored their brain waves during sleep. They found that people with a specific genetic variant showed altered brain wave patterns (faster sleep spindles) only when exposed to 3.6 GHz 5G radiation. This suggests that genetic differences may determine how sensitive individuals are to 5G's effects on brain activity during sleep.
Unknown authors · 2025
Researchers exposed 9-day-old chicken embryos to Wi-Fi radiation (2.4 GHz) for the entire incubation period and found cellular damage in developing kidney tissue. While overall organ development appeared normal, the radiation caused degenerative changes, increased cell death, and altered gene expression in the mesonephros (embryonic kidney). This suggests Wi-Fi radiation can disrupt normal tissue development even at low power levels.
Unknown authors · 2025
Temperature was the dominant factor, with optimal development at 30–32 °C. RF exposure at 18 GHz accelerated development at intermediate temperatures, but extreme RF and suboptimal temperatures prolonged development. Aedes aegypti was more sensitive to temperature changes than Aedes albopictus.
Dom NC, Dapari R, Halim NMHNA, Rahman ATA · 2025
Unknown authors · 2025
Researchers continuously exposed chicken embryos to Wi-Fi radiation at 2.4 GHz and 300 µW/m² for 9 and 14 embryonic days. Standard haematoxylin and eosin staining showed no notable differences in large blood vessel structure between exposed and control groups. Special staining methods revealed an adverse effect of Wi-Fi on the fibrous elements of the vessel wall. Elastic fiber optical density decreased significantly at both 9 and 14 embryonic days, while collagen fiber optical density increased at day 9 and decreased at day 14.
Levitt et al · 2025
De Borre E, De Massia C, Boone MN, Müller P, Thielens A · 2025
Unknown authors · 2025
Researchers evaluated the impact of 2.45GHz Wi-Fi radiation exposure for 4 hours daily over a consecutive 30-day period on neurobehavioral and brain oxidative stress parameters in adult zebrafish. Post-exposure assessments revealed significant neurobehavioral impairments, altered locomotion, decreased neurotransmitter levels (AChE), and increased oxidative stress.
Unknown authors · 2025
The study evaluated the in-vitro impact of simultaneous exposure to 5G-modulated 3.5 GHz and GSM-modulated 1.8 GHz signals on neuronal electrical activity, mitochondrial ROS production, and cellular stress protein responses. Primary cortical neurons and human immortalized skin fibroblasts were exposed at SARs of 1 or 4 W/kg for 15 minutes or 24 hours, respectively. No significant effects were found on the mean bursting rate or mean firing rate of cortical neurons, mitochondrial ROS production in fibroblasts remained unaffected, and HSF1 basal activity and responsiveness to MG132 showed no change. BRET assays detected only minor, inconsistent variations in RAS and PML basal activity and drug responsiveness, near the detection threshold.
Unknown authors · 2025
Researchers exposed newborn male Drosophila melanogaster to 3.5 GHz RF-EMF at 0.1, 1, and 10 W/m2 continuously throughout their life cycle in constant darkness. Metabolomic analysis after long-term exposure revealed disruptions in four metabolic pathways and 34 differential metabolites. Key metabolites including GABA, glucose-6-phosphate, AMP, N-formylglycinamide nucleotide, LPC, and MG showed significant decreases. The authors suggest that long-term electromagnetic wave treatment may reduce overall metabolic levels in fruit flies.
Gelenli Dolanbay E et al. · 2025
Pregnant Wistar Hannover rats were divided into sham control, full-gestation exposure (3T RFR), and late-gestation exposure (2T RFR) groups with 2 hours of exposure per day. Male offspring were euthanized at 12 months for testicular analysis. In the full-gestation exposure group, seminiferous tubule diameter and epithelial height were significantly reduced compared to controls. Abnormal sperm morphology increased significantly in the full-gestation exposure group. DNA damage markers and apoptosis were elevated in both exposure groups compared to sham.