3,138 Studies Reviewed. 77.4% Found Biological Effects. The Evidence is Clear.
All Exposure Types

Radio Frequency (RF)

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Radio frequency electromagnetic fields are produced by wireless communication devices and broadcast signals. They range from about 3 kHz to 300 GHz and include frequencies used by cell phones, WiFi, Bluetooth, and 5G. RF is typically measured in microwatts per square meter (µW/m²) or milliwatts per square centimeter (mW/cm²).

Concern Level Thresholds

Based on Building Biology Institute guidelines (µW/m² (microwatts per square meter)):

No Concern
< 0.1 µW/m²
Slight Concern
0.1 – 10 µW/m²
Severe Concern
10 – 1,000 µW/m²
Extreme Concern
> 1,000 µW/m²

See where common exposures fall on the scale:

Your RF Exposure in ContextA logarithmic scale showing your reading relative to Building Biology concern thresholds and FCC regulatory limits.Your RF Exposure in ContextNo ConcernSlightSevereExtreme0.1101,000FCC Limit 0.01100,000 uW/m2

Showing 376 studies with measured radio frequency (rf) exposure

Naltrexone pretreatment blocks microwave-induced changes in central cholinergic receptors.

Lai H, Carino MA, Wen YF, Horita A, Guy AW · 1991

Researchers exposed rats to microwave radiation at 2450 MHz (the same frequency as WiFi and microwave ovens) and found it altered brain receptors involved in memory and learning. When they gave the rats naltrexone (a drug that blocks opioid receptors) before exposure, it prevented these brain changes. This suggests microwave radiation affects the brain through the body's natural opioid system.

Oxidative Stress147 citations

Protective effects of melatonin and caffeic acid phenethyl ester against retinal oxidative stress in long-term use of mobile phone: A comparative study.

Ozguner F, Bardak Y, Comlekci S. · 2006

Researchers exposed rats to cell phone radiation (900 MHz) for 30 minutes daily over 60 days. The radiation caused significant oxidative damage to retinal tissue in the eyes. Two natural antioxidants, melatonin and CAPE, successfully protected against this damage, suggesting potential eye health risks from prolonged phone use.

Effect of chronic intermittent exposure to AM radiofrequency field on responses to various types of noxious stimuli in growing rats.

Mathur R. · 2008

Researchers exposed growing rats to amplitude-modulated radiofrequency radiation (similar to AM radio signals) for 2 hours daily over 45 days and tested their pain responses. The exposed rats showed altered pain processing - they became more emotionally reactive to sharp pain while experiencing less sensitivity to prolonged pain. This suggests that RF radiation can disrupt the nervous system's normal pain processing mechanisms during critical developmental periods.

Effect of chronic intermittent exposure to AM radiofrequency field on responses to various types of noxious stimuli in growing rats.

Mathur R · 2008

Researchers exposed growing rats to AM radio frequency fields (similar to some communication systems) for 2 hours daily over 45 days and tested their pain responses. The exposed rats showed altered pain processing - they became more emotionally reactive to short-term pain but less sensitive to long-term pain. This suggests that chronic RF exposure during development can rewire how the nervous system processes different types of pain signals.

Interaction of low level modulated RF radiation with Na+¯K+-ATPase.

Behari J, Kunjilwar KK, and Pyne S · 1998

Researchers exposed developing rats to radiofrequency radiation similar to what cell phones emit and found it significantly increased activity of a critical brain enzyme called Na+-K+-ATPase by 15-20%. This enzyme is essential for nerve cell function and brain development. The findings suggest that RF radiation can alter fundamental brain chemistry in developing animals, raising concerns about potential effects on brain development in children.

Modification of lethal radiation injury in mice by postradiation exposure to low-intensity centimeter-band radio frequency waves

Akoev IG, Mel'nikov VM, Usachev AV, Kozhokaru AF, · 1994

Researchers exposed mice to lethal doses of gamma radiation, then immediately treated them with low-intensity radiofrequency waves (2-27 GHz) for up to 23 hours. The RF-treated mice showed improved survival rates and lived longer than untreated mice. This suggests that certain RF frequencies might have protective biological effects under extreme conditions.

Interaction of microwaves and a temporally incoherent magnetic field on spatial learning in the rat.

Lai H. · 2004

Researchers exposed rats to microwave radiation at levels similar to cell phones and found it significantly impaired their ability to learn and remember spatial tasks. However, when they simultaneously exposed the rats to a weak, random magnetic field, it completely blocked the learning deficits caused by the microwaves. This suggests that certain types of magnetic field exposure might protect against microwave-induced brain effects.

Effects of exposure to low level radiofrequency fields on acetylcholine release in hippocampus of freely moving rats.

Testylier G, Tonduli L, Malabiau R, Debouzy JC · 2002

Researchers exposed freely moving rats to radiofrequency radiation at frequencies used by WiFi (2.45 GHz) and cell phones (800 MHz) to study effects on brain chemistry. They found that higher power exposures significantly reduced acetylcholine release in the hippocampus by 40-43%, a brain chemical crucial for memory and learning. The effects persisted for hours after exposure ended, suggesting that even brief RF exposure can disrupt normal brain function.

Uteroplacental circulatory disturbance mediated by prostaglandin F(2alpha) in rats exposed to microwaves..

Nakamura H, Nagase H, Ogino K, Hatta K, Matsuzaki I · 2000

Japanese researchers exposed pregnant rats to microwave radiation at 2.45 GHz (the same frequency as WiFi and microwave ovens) for 90 minutes and found it reduced blood flow to the placenta and increased stress hormones. The effects occurred at power levels too low to cause heating, suggesting the microwaves directly disrupted the pregnancy through biological mechanisms. This raises concerns about wireless device exposure during pregnancy.

Acute exposure to pulsed 2450-MHz microwaves affects water maze learning in the rat.

Wang, BM, Lai, H · 2000

Researchers exposed rats to pulsed microwave radiation at 2450 MHz (similar to WiFi frequency) for one hour before each training session in a water maze learning task. The microwave-exposed rats took longer to learn where a hidden platform was located and showed different swimming patterns compared to unexposed rats, indicating impaired spatial memory. This suggests that even brief microwave exposure can affect brain function and learning ability.

Melatonin and a spin-trap compound block radiofrequency electromagnetic radiation-induced DNA strand breaks in rat brain cells.

Lai, H, Singh, NP, · 1997

Researchers exposed rats to 2.45 GHz radiofrequency radiation (the same frequency used in microwave ovens and WiFi) for 2 hours and found it caused DNA strand breaks in brain cells. However, when they gave the rats either melatonin or a free radical scavenging compound before and after exposure, the DNA damage was completely blocked, suggesting that RF radiation damages DNA through free radical formation.

Naltrexone blocks RFR-induced DNA double strand breaks in rat brain cells.

Lai, H, Carino, MA, Singh, NP · 1997

Researchers exposed rats to microwave radiation at 2450 MHz (similar to WiFi frequencies) for 2 hours and found significant DNA damage in brain cells. When they gave the rats naltrexone, a drug that blocks the body's natural opioids, the DNA damage was partially prevented. This suggests that microwave radiation triggers the release of natural opioids in the brain, which then contributes to genetic damage.

Naltrexone blocks RFR-induced DNA double strand breaks in rat brain cells

Lai H, Carino MA, Singh NP · 1997

Researchers exposed rats to microwave radiation at 2.45 GHz for 2 hours and found significant DNA double strand breaks in brain cells. When they gave rats naltrexone (a drug that blocks the body's natural opioids), it partially prevented the DNA damage. This suggests that microwave radiation triggers the body's opioid system, which then contributes to genetic damage in brain tissue.

Naltrexone blocks RFR-induced DNA double strand breaks in rat brain cells.

Lai, H, Carino, MA, Singh, NP, · 1997

Researchers exposed rats to 2.45 GHz microwave radiation (the same frequency used in WiFi and microwave ovens) for 2 hours and found it caused DNA double strand breaks in brain cells. When they gave the rats naltrexone, a drug that blocks the body's natural opioids, it partially prevented this DNA damage. This suggests the body's own opioid system plays a role in how microwave radiation damages DNA in brain cells.

Single- and double-strand DNA breaks in rat brain cells after acute exposure to radiofrequency electromagnetic radiation

Lai H, Singh NP · 1996

Researchers exposed rats to radiofrequency radiation at 2450 MHz (similar to microwave oven frequencies) for 2 hours and found significant DNA damage in brain cells 4 hours later. Both single-strand and double-strand DNA breaks increased after exposure to radiation levels producing a whole-body SAR of 1.2 W/kg. This suggests that RF radiation can directly damage genetic material in brain tissue or impair the brain's ability to repair DNA damage.

Effect of 1.8 GHz radiofrequency electromagnetic radiation on novel object associative recognition memory in mice.

Wang K et al. · 2017

Researchers exposed mice to 1.8 GHz radiofrequency radiation (similar to cell phone signals) for 30 minutes and found it actually improved their memory performance on recognition tasks. The radiation changed brain cell structure and electrical activity in memory-related brain regions. However, the exposure level used was much higher than what people typically experience from everyday devices.

[Inhibitory effect of microwave radiation on proliferation of human pancreatic cancer JF305 cells and its mechanism].

Zhu W, Zhang W, Li Y, Xu J, Luo J, Jiang Y, Lu X, Lü S. · 2013

Researchers exposed human pancreatic cancer cells to microwave radiation at 2450 MHz (the same frequency used in WiFi and cell phones) for 20 minutes at various power levels. They found that the radiation inhibited cancer cell growth and triggered programmed cell death (apoptosis) through stress-related pathways. This suggests that microwave radiation can damage cellular functions even in cancer cells, which are typically more resilient than healthy cells.

Brain & Nervous SystemNo Effects Found

Cognitive function and symptoms in adults and adolescents in relation to rf radiation from UMTS base stations

Riddervold IS et al. · 2008

Danish researchers exposed 80 people (teenagers and adults) to cell tower radiation at 2.14 GHz for 45 minutes to test whether it affected their thinking abilities and caused symptoms. They found no significant impact on cognitive performance, though participants reported slightly more headaches during exposure compared to fake exposure sessions. The study suggests cell tower radiation at these levels doesn't impair mental function in the short term.

Structural and Ultrastructural Study of Rat Liver Influenced by Electromagnetic Radiation.

Holovská K et al. · 2015

Researchers exposed rats to 2.45 GHz microwave radiation (the same frequency used by WiFi and microwave ovens) for 3 hours daily over 3 weeks at power levels of 2.8 mW/cm². They found liver damage including inflammation, blood vessel dilation, and cellular changes including fat accumulation and dying liver cells. This suggests that chronic exposure to common microwave frequencies may harm liver function.

[Effect of modified SHF and acoustic stimulation on spectral characteristics of the electroencephalograms of the cat brain]

Ivanova VIu, Martynova OV, Aleinik SV, Limarenko AV. · 2000

Russian scientists exposed cats to 980 MHz electromagnetic fields and monitored their brain waves. The EMF exposure shifted brain activity patterns from high frequencies to lower ones, mimicking effects of sound stimulation. This suggests electromagnetic fields may affect the brain through the same pathways as acoustic signals.