Ma HR, Ma ZH, Wang GY, Song CM, Ma XL, Cao XH, Zhang GH. · 2015
Twenty adult male Sprague-Dawley rats were randomly divided into a normal group and a radiated group exposed to 900 MHz mobile phone radiation 4 hours per day for 18 consecutive days. Serum levels of ALT and AST did not change significantly in the radiated group compared to the normal group. Liver malondialdehyde content increased and glutathione content decreased in the radiated group. Histopathology revealed diffuse hepatocyte swelling, vacuolation, and focal necrosis. Bax protein expression was higher, bcl-2 protein expression was lower, and hepatocyte apoptosis rates were higher in the radiated group.
Kazemi E et al. · 2015
The study investigated the effect of 900 MHz GSM on oxidative stress and intracellular ROS levels in human mononuclear cells, monocytes, and lymphocytes. Blood samples from 13 healthy volunteers were exposed to RF radiation from a mobile phone simulator for 2 hours, while control samples were not exposed. Mononuclear cells were isolated by density gradient centrifugation and ROS was measured using a CM-H2DCFDA fluorescence probe and flow cytometry. Results showed a significant increase in ROS production in monocyte-rich cell populations after exposure, but no significant change in lymphocyte-rich populations.
Hancı H et al. · 2015
Pregnant Sprague-Dawley rats were divided into control and EMF groups, and the EMF group received 900 MHz EMF for 1 hour per day. Male pups were examined on postnatal day 21 for effects on spleen and thymus. In the thymus, malondialdehyde levels were significantly higher and glutathione levels significantly decreased in exposed pups compared to controls. In the spleen, malondialdehyde and glutathione levels increased while superoxide dismutase values decreased significantly. Transmission electron microscopy revealed pathological changes in cell morphology in both thymus and spleen tissues.
Malka N. Halgamuge, See Kye Yak and Jacob L. Eberhardt · 2015
Soybean seedlings pre-grown for 4 days were exposed for 2 h to GSM 900 MHz pulsed radiation or continuous wave radiation at amplitudes of 5.7 and 41 V m(-1) in a gigahertz transverse electromagnetic cell. Exposure to higher amplitude (41 V m(-1)) GSM radiation resulted in diminished outgrowth of the epicotyl. Exposure to lower amplitude (5.7 V m(-1)) GSM radiation did not influence outgrowth of epicotyl, hypocotyls, or roots. Higher amplitude CW radiation reduced root outgrowth while lower CW exposure reduced hypocotyl outgrowth. A separate 5-day exposure to 0.56 V m(-1) GSM radiation reduced epicotyl and hypocotyl growth but stimulated root outgrowth.
Furtado-Filho OV et al. · 2015
Researchers exposed young rats to 950 MHz ultra-high-frequency electromagnetic radiation to measure oxidative damage to DNA, proteins, and lipids in the left and right cerebral cortex. Radiation exposure lasted 30 minutes per day for up to 27 days, with a specific absorption rate ranging from 1.32 to 1.14 W/kg. In neonates, no modification of the biomarkers tested was detected, while 6-day-old exposed rats showed increased carbonylated proteins in the right cerebral cortex and decreased blood glucose. The researchers concluded that the elevated carbonylated proteins in the right cerebral cortex did not appear to be a consequence of oxidative stress.
Eris AH et al. · 2015
Researchers exposed ten male Wistar Albino rats to 900 MHz electromagnetic radiation at 15.14 V/m for 45 minutes and measured blood serotonin and glutamate levels before and after. Blood plasma serotonin and glutamate levels were measured using enzyme immunoassay. A single 45-minute exposure significantly increased blood serotonin levels but did not change glutamate levels.
Calvente I et al. · 2015
Researchers used spot measurements to characterize outdoor radiofrequency electromagnetic fields in the frequency range 100kHz-6GHz around the dwellings of 123 families from the INMA-Granada birth cohort in Southern Spain. The arithmetic mean electric field was 195.79mV/m and the mean power density was 799.01µW/m(2), with median values of 148.80mV/m and 285.94µW/m(2) respectively. All recorded values were well below International Commission for Non-Ionizing Radiation Protection reference levels. Exposure levels varied widely among different areas or towns/villages and also among seasons.
Marjanovic AM, Pavicic I, Trosic I, · 2015
Researchers evaluated the influence of a modulated radiofrequency field of 1800 MHz, strength of 30 V/m on oxidation-reduction processes within the cell. V79 cell line samples were irradiated for 10, 30, and 60 minutes, with a specific absorption rate calculated to be 1.6 W/kg. ROS levels significantly increased after 10 minutes of exposure, then decreased after 30 minutes, which researchers associated with antioxidant defence mechanism activation. Metabolic activity and total protein content in exposed cells did not differ significantly from control samples.
Hou Q, Wang M, Wu S, Ma X, An G, Liu H, Xie F · 2015
Researchers studied reactive oxygen species, DNA damage, and apoptosis in mouse embryonic fibroblasts (NIH/3T3) exposed to 1800-MHz GSM-talk mode electromagnetic radiation at an average specific absorption rate of 2 W/kg. Intracellular ROS levels increased significantly after exposure, reaching their highest level at one hour of exposure, then declining slightly with longer exposure up to eight hours. The percentage of late-apoptotic cells was significantly higher in the exposed group than in sham-exposed groups, while no significant effect on the DNA damage marker γH2AX was detected.
Cao H, Qin F, Liu X, Wang J, Cao Y, Tong J, Zhao H · 2015
Researchers exposed male Sprague Dawley rats to 1.8 GHz radiofrequency fields at different times of day to study whether circadian rhythms of plasma antioxidants were affected. Six groups of animals were exposed at a power density of 201.7 μW/cm2 and a specific absorption rate of 0.05653 W/kg for 2 hours per day for 32 consecutive days, each at a different time of day, while a seventh group served as sham-exposed controls. The circadian rhythms of melatonin, GSH-Px, and SOD were shifted in exposed rats, with levels significantly decreased when exposure was given at 23 and 3 h GMT. The researchers concluded that there may be adverse effects of radiofrequency exposure on antioxidant function, affecting both daily antioxidant levels and circadian rhythmicity.
Xiong L et al. · 2015
Researchers exposed 48 male Wistar rats to 30 mW/cm2 microwave for 10 min every other day for three times, and separately exposed PC12 cells to 30 mW/cm2 microwave for 5 min. Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. Significant changes in NMDA receptor subunits NR1, NR2A, and NR2B and related signaling molecules were observed, and these were closely associated with impaired synaptic plasticity.
Bodera P et al. · 2015
Researchers measured malondialdehyde (MDA), a marker of lipid peroxidation, in the blood, liver, kidneys, and brain of Wistar rats that were healthy or had inflammation, and exposed them repeatedly to 1800 MHz EMF. The exposure was repeated five times for 15 minutes and was studied both alone and combined with the opioid painkiller tramadol. MDA levels were slightly elevated in the blood, kidney, and brain of healthy rats in EMF-exposed groups treated with tramadol, while MDA in the liver remained at the same level across all groups. Overall, results for healthy rats and animals with inflammation did not differ significantly.
Kim BC, Kim W-K, Lee G-T, Choi H-D, Kim N, Pack J-K. · 2014
The study measured simultaneous human exposure to various radiofrequency signals at 1260 positions across Korea. The measured exposure levels were very low compared to international guidelines and Korean protection notice, with the highest total exposure ratio at 5.1 × 10(-3), approximately 7.1% of guideline limits.
Khalil AM, Abu Khadra KM, Aljaberi AM, Gagaa MH, Issa HS. · 2014
The study aimed to examine oxidant and antioxidant status in saliva of cell phone users by collecting samples before and after 15- and 30-minute calls. Saliva was analyzed for malondialdehyde, 8-oxo-dG, and antioxidant capacities using ORAC and HORAC assays. Mean 8-oxo-dG and protein concentrations peaked at 15 minutes, while HORAC, ORAC, and MDA levels increased progressively and reached maximum at 30 minutes. Talking time had no significant effect on 8-oxo-dG, MDA, ORAC, or HORAC levels. The authors concluded that there is no relationship between radiofrequency radiation exposure and changes in the salivary oxidant/antioxidant profile.
Shirai T et al. · 2014
Researchers exposed rats to 2.14 GHz W-CDMA signals for 20 hours a day over three generations. No abnormalities were observed in mother rats or offspring across any biological parameters including neurobehavioral function. The study used three SAR levels: high (<0.24 W/kg), low (<0.08 W/kg), and sham exposure. Pregnant rats were exposed from gestational day 7 to weaning, and offspring were continuously exposed until 6 weeks of age.
Kang KA et al. · 2014
Researchers exposed U87, PC12, and SH-SY5Y neuronal cells to combined radiofrequency radiation at 837 MHz CDMA and 1950 MHz WCDMA with a specific absorption rate of 2 W/kg each for 2 hours. They also tested co-exposure with hydrogen peroxide or menadione and measured intracellular reactive oxygen species using flow cytometry with a fluorescent probe. Combined RF radiation alone did not consistently affect ROS levels in a time-dependent manner, and co-exposure with hydrogen peroxide or menadione showed no statistically significant change compared to the chemicals alone.
Fasseas MK et al. · 2014
Researchers exposed synchronized populations of C. elegans at different developmental stages to radiation from GSM mobile phones, Wi-Fi routers, and DECT phones at electric field levels below ICNIRP guidelines. They examined wild-type and aging- or stress-sensitive mutant worms for changes in growth, fertility, lifespan, chemotaxis, short-term memory, ROS production, and apoptosis using fluorescent markers and qRT-PCR. No statistically significant differences were found between exposed and control animals in any of the measured outcomes.
Sepehrimanesh M et al. · 2014
Twenty adult male Sprague-Dawley rats were exposed to 900 MHz RF-EMF for 1, 2, or 4 hours per day over 30 days, with a sham-exposed control group. At the end of the experiment, the long-time exposure group showed significantly higher FSH and LH levels than the sham group. The long-time exposure group also had significantly increased activin B and prolactin, decreased inhibin B compared to sham and short-time exposure groups, and decreased testosterone compared to short- and moderate-time exposure groups.
Geronikolou S et al. · 2014
Egg laying in the second generation was compared between exposed and non-exposed cultures using advanced statistics. The 900 MHz exposure showed statistical significance, while the cordless phone exposure showed limited statistical significance. The researchers also calculated and simulated the near-field radiation patterns inside the vials. They found that the power difference between the two fields becomes null when the cylinder radius and antenna height increase.
Balakrishnan K et al. · 2014
The study aimed to measure serum HSP70, CRP, and hsp70 gene expression in frequent mobile phone users. The researchers enrolled 120 frequent users and 102 infrequent users as controls. Serum HSP70 and CRP were measured by ELISA and hsp70 gene expression by RT-PCR. Frequent users had significantly higher serum HSP70 and CRP concentrations and higher hsp70 gene expression than infrequent users. Serum HSP70 concentration was independent of exposure duration, whereas CRP increased with duration.
Azadi Oskouyi E et al. · 2014
In this study, 18 male rabbits were randomly divided into two experimental groups and one control group, and the experimental groups received simulated microwaves at 950 MHz with output power of 3 and 6 watts for 2 weeks, 2 h a day. The epithelial height and diameter of the epididymis decreased significantly in both the 3 watt and 6 watt groups, while testosterone level decreased significantly only in the 6 watt group. The rate of apoptosis in the epithelial cells of the epididymis increased significantly only in the 6 watt group.
Tök L, Nazıroğlu M, Doğan S, Kahya MC, Tök O. · 2014
Thirty-two rats were randomly assigned to four groups, with one group exposed to Wi-Fi at 2.45 GHz for 60 minutes daily over 30 days and another group receiving melatonin during the same exposure. Lipid peroxidation was slightly higher in the Wi-Fi group than in controls, and glutathione peroxidase activity was significantly lower in the Wi-Fi group. Melatonin supplementation significantly decreased lipid peroxidation and increased both glutathione peroxidase activity and reduced glutathione levels compared with the Wi-Fi group.
Ragy MM. · 2014
Researchers exposed male rats to 900-MHz electromagnetic radiation for one hour daily over 60 days, with a withdrawal group that had a 30-day recovery period. Exposure significantly increased malondialdehyde levels and decreased total antioxidant capacity in brain, liver, and kidney tissues. Serum levels of alanine transaminase, aspartate aminotransferase, urea, creatinine, and corticosterone were significantly increased, while catecholamines were not significantly changed. All these alterations were corrected after the withdrawal period.
Hässig M et al. · 2014
The study aimed to observe the influence of non-ionizing radiation from base stations on redox protein activity in bovines. The experiment found that NIR changed enzyme activities, with certain enzymes disabled and others enabled. Individual cows showed varied reactions, with some reacting to NIR, others not reacting, or reacting inversely. The study approach of testing ten cows each ten times during three phases was deemed appropriate. The authors noted that future studies should set the post-exposure phase later.
Valbonesi P, Franzellitti S, Bersani F, Contin A, Fabbri E. · 2014
Researchers exposed rat PC12 cells to 1.8 GHz continuous wave or GSM-modulated signals at a SAR of 2 W/kg for 4, 16, or 24 hours. HSP70 gene expression was measured by RT-PCR, while HSP70 protein and MAPK phosphorylation were measured by Western blotting. Only the GSM-217Hz signal increased HSP70 transcription after 16 or 24 hours of exposure. HSP70 protein levels and three MAPK signaling pathways were unchanged by any of the signals.
